Effects Of Lactate Dehydrogenase Inhibitor Galloflavin On The Proliferation,Migration And Invasion Of Lung Adenocarcinoma A549 Cells And Their Mechanisms

Objective To observe the effects of different concentrations of lactatedehydrogenase inhibitor Galloflavin(GF)on the proliferation,migration and invasion of lung adenocarcinoma A549 cells,and to preliminarily explore their mechanisms.Methods The lung adenocarcinoma A549 cells were cultured in vitro,and the cells were divided into control group(no GF treatment group),and the experimental group(GF treatment group,concentration was 12.5,25,50,100 ?mol/L,respectively),after24 and 48 hours,respectively.The proliferation inhibition rate of GF on A549 cells was detected by CCK-8 method.A549 cells were treated with different concentrations(0,25,50,100 ?mol/L)GF.After 24 hours,the effect of GF on the migration ability of A549 cells was detected by scratch test.The effect of GF on the migration ability of A549 cells was detected by Transwell assay.The lactate dehydrogenase activity assay kit was used to detect the effect of GF on lactate dehydrogenase activity;the lactic acid quantitative kit was used to detect the effect of GF on the amount of lactic acid production;The effect of GF on the expression of E-cadherin(E-cad)and vimentin(VIM)in supernatant and A549 cells was detected by ELISA.Results The results of CCK-8 showed that the inhibition rate of A549 cells between the control group and the experimental group was significantly different(P<0.05).The proliferation of the experimental group was observed at the same time(24,48 h).The inhibition rate was higher than that of the control group.With the increase of GF concentration,the inhibition rate of proliferation increased,the difference was statistically significant(P<0.05).At the same GF concentration(0,12.5,25,50,100?mol/L),48 The proliferation inhibition rate of h was higher than 24 h,and the difference was statistically significant(P<0.05).The results of scratch test showed that the cell migration rate of the experimental group was lower than that of the control group(P<0.05).With the increase of GF concentration,the mobility decreased gradually,and the difference was statistically significant(P<0.05).The results of Transwell showed that the number of invading cells in the experimental group was smaller than that in the control group.With the increase of GF concentration,the number of invasive cells decreased gradually,and the difference was statistically significant(P<0.05).The detection of lactate dehydrogenase activity showed that the activity of lactate dehydrogenase in the experimental group was significantly lower than that in the control group.With the increase of GF concentration,the activity of lactate dehydrogenase decreased gradually,and the difference was statistically significant(P<0.05).The results of lactic acid test showed that the amount of lactic acid in the experimental group was significantly lower than that in the control group.With the increase of GF concentration,the amount of lactic acid was gradually decreased,and the difference was statistically significant(P<0.05).The results of ELISA showed that compared with the control group,the concentration of E-cad protein in the supernatant of the cell culture supernatant of each concentration of GF intervention group was significantly increased,and the concentration of E-cad protein in the supernatant of the cell increased with the increase of GF concentration.Gradually increased,the difference between the intervention groups was statistically significant(P<0.05),and the intracellular E-cad protein concentration gradually increased.The difference between the intervention groups was statistically significant(P<0.05).Compared with the control group,the concentration of VIM protein in the supernatant of the cell culture supernatant of each concentration of GF intervention group was significantly decreased,and with the increase of GF concentration,the concentration of VIM protein in the supernatant of the cell gradually decreased,between the intervention groups.The difference was statistically significant(P<0.05),and the intracellular VIM protein concentration also decreased gradually.The difference between the intervention groups was statistically significant(P<0.05).Conclusion GF can inhibit the proliferation,migration and invasion of A549 cells in vitro,which may be due to inhibition of aerobic glycolysis and epithelial-mesenchymal transition.