Analysis Of Molecular Pathology And Clinical Features Of 59 Patients With Melanoma

Objective:Melanoma(MM)has become one of the fastest growing malignant tumors with an annual growth rate of 3%-5%.The incidence of melanoma in China is about 0.55/100,000,but the mortality rate accounts for about 75% of skin malignancies.Although molecular targeted therapy has been widely used in the treatment of advanced melanoma in recent years,the efficacy is still unsatisfactory.Therefore,understanding its molecular pathological characteristics and finding specific genetic mutations are important for improving the therapeutic effect and prolonging the survival period.In recent years,the development of gene detection is changing with each passing day,and the use of this technology to study the genetic mutation characteristics of rare and rare tumors is gradually used to provide reference for molecular targeted therapy.This study collected data on the detection of all exon of 11 cancer-related genes in tumor tissues by gene detection in Northeast China,obtained the characteristics of gene mutations,and carried out survival analysis,in order to guide MM diagnosis and molecular targeted therapy according to the characteristics of gene mutation.To provide new ideas for the diagnosis and treatment of the disease.Materials and Methods:The subjects were 59 patients who underwent surgical resection and pathological diagnosis of MM from June 2013 to June 2018.The follow-up period was successful.The last follow-up time was January 1,2019.Detailed clinical data of the above patients and detailed genetic mutation data obtained by performing gene detection sequencing of 11 cancer-related gene exons in the above-mentioned patients were collected.The data was sorted,screened and statistically analyzed with SPSS20.0 software.The count data was recorded in the form of N(%),and the measurement data was recorded in the form.The survival curve was drawn by Kaplan-meier method,the median survival time was calculated and the is proportional risk hypothesis was verified.The log-rank test was used to compare the difference between the general data and the clinical data,and the meaningful variables were included according to the log-rank test results.Multivariate Cox regression analysis to explore independent factors influencing patient survival outcomes.The test level was 0.05,and the difference was statistically significant at P < 0.05.Results:There are 11 oncogene exons in this study,including B-RAF15,N-RAS2,N-RAS3,c-kit9,c-kit11,c-kit13,c-kit17,c-kit18,PDGFR12,PDGFR14,PDGFR18.There were 7 mutations found in 59 cases of MM,including 12 cases of B-RAF15 mutation,2 cases of N-RAS3 mutation,2 cases of c-kit13 mutation,2 cases of c-kit18 mutation,1 case of c-kit11 mutation,c-There was 1 case of kit17 mutation and 1 case of PDGFR18 mutation.Kaplan-Meier single-factor survival analysis(Log-rank test)found that NRAS,cKIT gene mutation,gender,age and MM patients were associated with ovearall survival(OS)(P<0.001,P=0.004,P=0.033).,p=0.020).However,there were no significant correlations between clinical features such as tumor thickness,tumor location,presence or absence of ulcers,and TNM staging(P>0.05).Multivariate analysis using COX proportional hazards regression model showed that NRAS and cKIT gene mutations were independent influencing factors of MM overall survival.Concusions:(1)MM patients with gender,age,NRAS,cKIT gene mutation and OS have correlation,tumor thickness,tumor location,presence or absence of ulcer,T stage,N stage,M stage,clinical stage,pathological type,treatment,BRAF gene Mutations and the like have no significant correlation with OS.(2)NRAS,cKIT gene mutation is an independent influencing factor of MM overall survival.(3)Using gene detection,we can find that MM has the characteristics of characteristic gene mutation.In the future,MM diagnosis and molecular targeted therapy may be guided according to the characteristics of gene mutation,which provides a new idea for the diagnosis and treatment of the disease.