Effects Of Xiaoxian Xiong Decoction On Lipid Metabolism And Expression Of Related Transporters Protein In Apolipoprotein E-Deficient Mice

Objective:An important pathological basis for cardiovascular disease is atherosclerosis?AS?,which is one of the risk factors for human death.Its formation is inseparable from the disorder of lipid metabolism.At present,the clinical use of statins lipid-lowering drugs has limitations due to side effects such as liver and kidney damage.Therefore the prevention and treatment of atherosclerosis by traditional Chinese medicine has become a hot spot in the field of global medicine.To explore the effect of TCM,modified Xiaoxian Xiong Decoction on atherosclerosis and the plausible mechanism of anti-atherosclerotic effect though studying the effects of Xiaoxian Xiong Decoction on the serum lipid spectrum,and related protein factors of Apolipoprotein E-Deficient mice Based on Syndromes and Treatment.Methods:Adaptive support of Apo E^-/-mice for 7 days,divided into two groups,one set up as a blank control group,feeding ordinary feed;The other group was set up as a building module and was fed with high-fat feed for 28 days?28 days later,two mice were randomly selected for each of the blank control groups and the building module?.A mouse model of atherosclerosis was established by observing changes in blood lipids in the mice,pathological sections of the aorta and heart,and oil red O staining.._therosclGive atorvastatin and Xiaoxian Xiong Decoction for 4 weeks respectively.The feeding was terminated after 8 weeks,and all groups of mice were subjected to blood anatomy.The blood lipid content?TG,TC,HDL-C,LDL-C?of each group was measured by a blood biochemical analyzer.The lipid accumulation plaques in the aortic valve and aorta of each group were observed by oil red staining.The morphology and structure of the liver of each group were observed by HE staining.The expression of intrahepatic lipid transporter ABCA1/G1 in each group was determined by immunoblotting.Results:?1?The red O staining results of aortic valve oil showed that the model was established successfully;?2?Xiaoxian Xiong Decoction can significantly reduced the serum lipid levels?including TG,TC,LDL-C?;?3?Oil red O staining results indicate Xiaoxian Xiong Decoctiont can significantly reduce lipid plaques in the aortic valves of ApoE^-/-mice;?4?Oil red O staining results show Xiaoxian Xiong Decoction significantly alleviate lipid plaque deposition in the aortic arch of ApoE^-/-mice;?5?Xiaoxian Xiong Decoction can reduce the fat accumulation in the liver;?6?Xiaoxian Xiong Decoction can increase the expression of ABCA1 and ABCG1 in liver;Conclusion:Xiaoxian Xiong Decoction can reduce serum lipids and aortic plaque deposition,increase the expression of liver ABCA1 and ABCG1 effectively in the ApoE^-/-mice and may promote the reverse transport of cholesterol,mitigating atherosclerotic lesions.