| Background Cardiovascular disease is increasingly being valued,and half of all cardiovascular disease cases are estimated to occur in Asia.Cardiovascular disease in Asia is the World Health Organization's key concern.According to the statistics of China's current cardiovascular disease in thenumber of about 290 million,resulting in a severe public health burden form.In 2002,the number of deaths due to coronary heart disease in China exceeded 700,000 [1].Since the reform and opening up,the incidence and mortality of coronary heart disease in China have been more than a year,especially inrural areas [2,3].The absolute number of coronary heart disease events and deaths is expected to increase sharply between 2010 and 2029[4].Coronary heart disease is,a common type of cardiovascular disease,caused by endothelial celldamage,oxidized low-density lipoprotein deposition resulting in coronary atherosclerotic plaque formation,resulting in coronary artery blood flow is not smooth,insufficient blood supply,Severe vascular occlusion causes heart disease caused by myocardial injury or necrosis caused by intravascular thrombosis.And coronary heart disease without early warning and requiring lifelong medication.Because there is currently nobiochemical index for unstable anginapectoris(UAP)can be used in clinical adjuvant testing,it is difficult to do early accurate diagnosis,only according to"characteristic chest pain symptom","ECG dynamic evolution" and other subjective strong,specificity and low sensitivity of traditional indicators to make clinical suspect diagnosis,and at this stage as the diagnostic gold standard "Coronary angiography is delayed in clinical practice because of its invasive risk,technical difficulty and high cost.In order to realize the early diagnosis and differential diagnosis of unstable anginapectoris,and to evaluate the risk and prognosis accurately,it is urgent to find new molecular markers with specificity and sensitivity in diagnosis and prognostic judgment,and to discuss the possible regulation mechanism.Objective This topic mainly discusses the difference of serum mi R-499a-3p between serum mi R-499a-3p of unstable anginapectoris patients and non coronary heart disease patients with suspected angina pectoris,and further studies its ADAM10 effect on downstream target gene.To provide a scientific basis for further study of serum mir-499a-3p as a potential biological marker for UAP patients,and to provide a potential intervention target for avoiding the occurrence of malignant coronary events.In order to further explore its regulative effect on downstream target gene ADAM10 and its effect on the proliferation,migration and apoptosis of endothelial cells and vascular smooth muscle cells through downstream target gene ADAM10,he preliminary experimental basis was provided.Methods1.We collected 5 ml of peripheral blood from patients with UA in the experimental group(n=54)and non-coronary heart disease patients(n=47)with suspected angina in the control group to obtain total serum RNA.2.The relative expression levels of mi R-499a-3p in the UA group and non-CHDpatients with suspected angina were determined.3.To verify the downstream target gene ADAM10 of mir-499a-3p.After the target Gene 3 '-UTR region was constructed to the luciferase of the reported gene,the changes in gene expression could quantitatively reflect mi RNA inhibition of the target gene by comparing the expression of mi RNA.Results 1.The relative expression of serum mi R-499a-3p inexperimental group and control group was obtained by relative quantitative analysis,and the expression of serum mi R-499a-3p in UAP group increased significantly(p<0.05)compared with control group.2.mi R-499a-3p binds to the 3'-UTR of downstream target gene ADAM10 and inhibits its expression.Conclusion The serum level of mi R-499a-3p in UAP patients with non-coronary heart disease increased,which may provide a new strategy for the effective detection of UAP.Serum mi R-499a-3p can regulate the expression of ADAM10. |
PDF Full Text Request