| Rcent years,with the developing of the society and lifestyle,the mobility of the diabetes has an upward trend.serious threats people’s quality of life.The current mostly therapeutic methods are Hypoglycemin drugs and Insulin injection.Rubus suavissimus S.Lee as a crude plant which has the function of decreased blood glucose levels,has been used for years.This article has mainly studied what main components can reduces the blood sugar in the Rubus suavissimus S.Lee.1.Guangxi sweet tea as raw material,was roughly extracted by water extraction and ethanol extraction.The content of sweet tea glycosides in water and ethanol extracts of Guangxi sweet tea was determined by high performance liquid chromatography.The content of sweet tea glycosides in four producing areas including Jinxiu,Xinping,Pulu and Lipu sweet tea was also determined.The results showed that the concentration of water extract and ethanol extract of Guangxi sweet tea（Lipu origin）were 1.46 mg/g,3.33 mg/g,14.34 mg/g and15.64 mg/g respectively（the corresponding extraction solvents were water,30%ethanol,50%ethanol and 75%ethanol respectively）.The content of sweet tea glycoside in Jinxiu,Xinping and Pulu were 21.76 mg/g,69.01 mg/g and 23.79mg/g respectively（the extraction solvent was 95%ethanol）.Alcohol).The content of sweet tea extracted by 75%ethanol was the highest in the ethanol extracts of different concentration in Lipu producing area.It was concluded that the higher the concentration of ethanol,the higher the content of sweet tea extract.2.The ethanol extract of sweet tea from Guangxi was partially dissolved in water and then filtered onto 101 macroporous resin columns.Gradient elution was carried out with 30%,50%and 75%ethanol,respectively,to obtain the total extract of each part of sweet tea.We used petroleum ether,ethyl acetate and n-butanol separately to extract,then passed silica gel column chromatography,gel column,polyamide column and recrystallization.Analysis by spectral identification,We can isolate four compounds from 75%ethanol extract,including Rubusoside,Quercetin-3-beta-D-galactoside,Kaempferol,Sucrose。3.Objective:To study the effects of ethanol extract of sweet tea from Guangxi on blood sugar and glucose tolerance in normal mice and diabetic mice,respectively.METHODS:Normal KM mice were divided into four dosage groups:blank group,high,medium and low ethanol extracts of sweet tea from Guangxi（250,150,100 g kg-1）,and were given orally for 40 days to observe their effects on blood sugar of normal mice.Glucose tolerance was measured every 10 days.One day after fasting,10%glucose solution was intraperitoneally injected into each group.The blood glucose values before（0 h）glucose injection and 0.5,1 and 2 h after（0.5,1 and 2 h）glucose injection were measured by blood glucose meter and blood glucose test paper respectively.Diabetic mice experiment:Preliminary study on hypoglycemic effect of ethanol extracts of sweet tea from Guangxi on KM mice model of type 2 diabetes induced by intravenous injection of streptozotocin STZ.100 SPF KM mice were randomly divided into model group,metformin group(0.25 g kg-11 d-1),sweet tea 30%,50%and 75%ethanol extracts at different concentrations were divided into high,middle and low dose groups.Ten other mice were taken as normal group and given orally for 4 weeks,and blood sugar was measured once a week.Observe general indexes such as body weight,hair and so on.The results showed that the low-dose group and the middle-dose group of ethanol extract of sweet tea in Guangxi had significant effects on the fasting blood glucose of normal mice,and with the increase of administration time,it inhibited the hyperglycemia induced by glucose in mice（P<0.01,P<0.05）.At the same time,compared with the blank control group,the body weight of low-dose group and middle-dose group decreased significantly after 40 days of administration.The content of Sweetenin extracted by 75%absolute ethanol was the highest in all ethanol extracts,and 75%ethanol extracts could significantly reduce blood sugar in mice.It is concluded that the extract of sweet tea can reduce blood sugar in diabetic mice,and its mechanism may be related to the antioxidant capacity of liver.4.The research method of Hypoglycemic effect of water extract of sweet tea in Guangxi.We preliminarily studied the hypoglycemic effect of water extract of sweet tea in Guangxi through the model of type 2 diabetes mellitus in KM mice induced by intravenous injection of streptozotocin STZ.Fifty SPF KM mice were randomly divided into model group,metformin group(0.25g kg-11 d-1),high,medium and low dose groups of sweet tea water extract.In addition,10mice were taken as normal group and given orally for 4 weeks.Blood sugar was measured once a week.In the fourth week,liver serum was taken to detect hepatic muscle glycogen,SOD（superoxide dismutase）,MDA（malondialdehyde）and other indicators to preliminarily determine the hypoglycemic effect of Guangxi sweet tea water extract.Results show that compared with the blank group,the FBG level in the model blank group was higher,the MDA level in liver tissue was higher,and the levels of hepatic glycogen and SOD were lower（P<0.05）,and compared with the model group,the FBG level in the water extract group and the positive metformin group decreased significantly,SOD activity increased,hepatic glycogen content increased and MDA content decreased,with significant statistical differences（P<0.05）,it is suggested that liver tissue enhances its antioxidant capacity.We can conclude that sweet tea extract can reduce blood sugar in diabetic mice,and its mechanism may be related to the antioxidant capacity of the liver.5.Preliminary analysis of migration components in blood of ethanol extract of sweet tea from Guangxi.Guangxi sweet tea was extracted by 95%ethanol for3 times,1 h each time.After being concentrated and concentrated,95%sweet tea extracts from Guangxi sweet tea were extracted.Then 95%alcohol extracts were removed by 85%methanol washing and then removed by HPLC-MS.In addition,5 SD rats were divided into blank control group and 95%ethanol extract group.They were fasting for 12 hours and weighed.According to 1.5ml/100g,distilled water and 95%ethanol extract solution were given to the stomach respectively.Except blank group,95%ethanol extract group took blood at 30,90,150,210 minutes and 4000 rmp centrifugation for 10 minutes respectively.Serum samples were separated and packed.The optimal time of blood collection was determined by HPLC-MS analysis after protein precipitation.The chemical constituents of ethanol extract of sweet tea in Guangxi were analyzed by HPLC-MS in vitro and in vivo.On this basis,the chromatograms of ethanol extract of sweet tea in Guangxi and serum samples were compared and analyzed.According to the retention time of each component,the prototypecomponents and metabolic transformation components entering the blood were determined. |
PDF Full Text Request