Association Of The XKR6 Rs7014968 SNP And The Risk Of Coronary Artery Disease And Ischemic Stroke

OBJECTIVE:Cardiovascular and cerebrovascular diseases account for the largest proportion of deaths due to illness worldwide.China will inevitably enter the elderly society in the future,and the prevention and treatment of coronary artery disease(CAD)and ischemic stroke(IS)are not optimistic.The above diseases belong to diseases of different systems of the human body.However,there is a large part of the crossover risk factors and pathophysiology.Foreign studies have shown that the single nucleotide polymorphism(SNP)of the rs7819412 on the XKR6 gene is associated with human lipid metabolism,while the rs7014968 SNP belongs to the same gene.This paper aims to explore the association between the XKR6 rs7014968 SNP and serum lipid levels and the susceptibility to CAD and IS in the Guangxi Han population.METHODS:In this study,Han residents living in Guangxi Zhuang Autonomous Region were selected as study subjects.They were divided into control(n=624)and case groups.The case group was also divided into CAD(n=588)and IS(n=544)subgroups.Questionnaires were used to collect their relevant information and clinical data.Comprehensive physical examination was performed.Fasting venous blood specimen for blood biochemical examination and extraction of whole blood DNA was obtained after 12 hours of fasting.Genotyping of the XKR6 rs7014968 in the samples was performed using the Snapshot technology.RESULTS:1.Serum total cholesterol(TC)levels in the control group were significant difference among the XKR6 rs7014968 genotypes(P<0.05),and serum TC levels were significantly higher in the XKR6 rs7014968C allele carriers than the allele non-carriers.2.The XKR6 rs7014968C allele carriers had higher risk of CAD(CT genotype:OR=1.418,95%CI=1.028-2.046,P=0.042;CC genotype:OR=6.087,95%CI=1.835-10.600,P=0.003).3.The XKR6 rs7014968C allele carriers were also associated with increased risk of IS(CT genotype:OR=1.589;95%CI=1.087-2.323,P=0.017;CC genotype:OR=5.364,95%CI=1.571-18.313,P=0.007).After the study samples were adjusted for age,BMI,smoking and hypertension,the results in the genetic model still showed that individuals carrying the rs7014968CT/CC genotypes were associated with the risk of CAD and IS(P<0.05).4.Hierarchical analysis also showed that the subjects with rs7014968C allele in women,those older than60 years,those with body mass index(BMI)>24 kg/m~2,and those with hypertension had an increased risk of CAD(P<0.05).5.The subjects with rs7014968C allele in males,BMI>24 kg/m~2,smokers,and those with hypertension also had a increased risk of IS(P<0.05).Conclusion:The XKR6 rs7014968 SNP was significantly associated with serum TC levels in healthy control group,and the rs7014968C allele carriers had higher TC levels than the rs7014968TT homozygotes.The XKR6 rs7014968SNP was associated with the occurrence of CAD and IS in Guangxi Han residents,and the risk of CAD and IS in the rs7014968C allele carriers was significantly increased.The XKR6 rs7014968 SNP may also interacted with age,gender,smoking,BMI and high blood pressure to affect the risk of CAD and IS in the Guangxi Han residents.