Correlation Of High Intensity Swimming,voluntary Exercise And Telomere Length In Rats

Objective: There are different views on the telomere length of the body effected by exercise.This study intends to explore the relationship between exercise and telomere length by observing the oxidative stress process,inflammatory reaction and telomerase mechanism in the body.Aspects provide theoretical data on the relationship between exercise and health.At the same time,however,sports have a high degree of voluntary factors.Some people like to engage in sports activities and are highly voluntary.There is also a large proportion of people who are naturally not interested in exercise,that is,low-volume voluntary.Therefore,when studying the effect of exercise on the telomere length of the body,it is necessary to consider the psychological stress of telomere in the exercise of low-volume voluntary rats.The effect of length,so this paper will study and analyze the relationship between exercise,voluntary exercise and telomere length.Methods: Sixteen SD rats aged 7 weeks were selected for study.The voluntary degree of exercise was obtained by spontaneously rotating cages.The rats were divided into two groups: exercise group and static group by stratified random sampling.The exercise group performed a 5-week high-intensity load swimming exercise.In the first week,there was no load in the tail.In the second week,the tail was loaded with 5%.At the 3-5th week,the tail weight was 10%,30 min/day,and 5 days/week.The changes of SOD,MDA,IL-6,TNF-?,p16 gene expression,telomerase activity and telomere length were analyzed,and the correlation between voluntary activity and telomere length was analyzed.The analysis used independent sample t-test and Pearson correlation.Results: Compared with the control group,there was no significant change in serum SOD in the exercise group(P=0.109),MDA was significantly decreased(P=0.001),and SOD/MDA was significantly increased(P=0.01).Compared with the control group,there was no significant change in serum IL-6 in the exercise group(P=0.638),and there was no significant change in TNF-?(P=0.627).Compared with the control group,the expression of p16 gene was significantly decreased in the exercise group.P=0.001),telomerase activity was significantly increased(P=0.02).Compared with the control group,there was no significant change in leukocyte telomere length in the exercise group(P=0.1).There was no correlation between telomere length(r=-0.393,P=0.44),and there was a positive correlation between voluntary activity and telomere length in exercise group(r=0.926,P=0.008).Conclusion: 1.5 weeks of intense swimming can reduce the body's oxidative stress levels.2.The 5-week high-intensity swimming exercise can increase telomerase activity and inhibit the expression of p16 gene,which protects telomeres.3.The more voluntary the rat's movement during exercise,the more effective it is to delay the shortening of telomere length.