Component Analysis And Metabolomics Study Of Fufang Kushen Decoction Against Ulcerative Colitis

Ulcerative colitis(UC)is a chronic non-specific inflammatory disease of the colon and rectum with unclear etiology.Its etiology and pathogenesis are complex,which is currently believed to be caused by the interaction of environment,heredity,immunity,diet,spirit,medication and other factors.Its incidence of a disease is high,this disease can happen at any age,but come on in the crowd of 20-40 years old more,it is the common difficult disease of gastroenterology.Fufang Kushen Decoction(FFKSD)is an effective clinical prescription for the treatment of UC by professor Fan Heng from the combination of Chinese and western medicine in the affiliated union hospital of tongji medical college of huazhong university of science and technology.FFKSD is composed of Chinese herbs such as radix sophorae flavescens,radix sanguisorbae,rhizoma bletillae,radix glycyrrhizae and indigo naturalis.it has the effect of clearing heat,detoxifying,dampness,cooling blood and stopping bleeding.In the long-term clinical practice and experimental study showed that the FFKSD can remove the large intestine of damp and hot dirty evil,promote the healing of intestinal mucosal erosion,ulcer,repair the intestinal mucosal barrier,reduce the inflammatory reaction,improve the body’s immune function low status,clinical treatment,3000 patients with UC,curative effect is distinct,efficient above 96%,and its curative effect is superior to the similar clinical commonly used traditional Chinese medicine.But,FFKSD what material is effective,its cure ulcerative colitis why effective all do not know.This thesis adopts modern instrumental analysis technique by means of interdisciplinary research method,and studied the pharmacodynamic material basis of the empirical prescription.The metabolomics of FFKSD treatment of ulcerative colitis in rats was preliminarily studied too.The compound matrine,saponins,phenolic acids and other types of chemical components in the FFKSD were analyzed and identified by using LC-MS/MS.27 compounds including matrine,oxymatrine,sophordine,glycyrrhizin,glycyrrhizin,glycyrrhizin,indigo and indirubin were identified.In the same way,the LC-MS/MS was used to study the blood-entering components of FFKSD.Comparing the mass spectra of serum,blank serum and FFKSD after rat administration,different compounds were found in positive and negative ion mode.In positive ion mode,it was found that there were 21 differences between the serum of rats in the FFKSD group and the serum of the blank group.Analysis found that six substances may be directly into the blood,including matrine,oxymatrine and glycyrrhizin.In the negative ion mode,it was found that there were 8 differences in serum between the rats in the FFKSD group and the rats in the blank group.Two substances were found to be direct into blood,including glycyrrhizin.In the pattern diagram of serum positive and negative ions,the unidentified transitional components may be metabolites of the effective components of FFKSD.Based on the study of the chemical components and blood components of the extract of FFKSD,metabolomics method was used to study the mechanism of FFKSD in the treatment of UC rat model.The mechanism of FFKSD in the treatment of UC was explored from the perspective of metabolites.In the metabolomics study of serum and urine in a rat model of UC,26 potential biomarkers were found in the experiment,many metabolic pathways were involved,such as thiamine metabolism,arachidonic acidmetabolism and TCA cycle.In the rat model of UC,the levels of these potential biomarkers were up-regulated or down-regulated in different degrees compared with the normal blank group,while the content of FFKSD administration group showed different degrees of callback compared with the normal blank group.In the metabolomics study of colon,kidney,and liver tissue in a rat model of UC.A total of 13 potential biomarkers were discovered in the experiment,affecting multiple metabolic pathways such as amino acid metabolism and glutathione metabolism.In the rat model of UC,the levels of these potential biomarkers in each tissue were up-regulated or down-regulated to varying degrees relative to the normal blank group.The content of the FFKSD administration group also showed different degrees of callback compared with the model group.The results showed that FFKSD in the treatment of UC was effective through a variety of metabolic pathways,thus providing a modern scientific basis for the effective treatment of ulcerative colitis by this clinical experience.