The Relationship Between The Signaling Pathway And The Expression Of Vascular Endothelial Growth Factor In The Cultured Human Retinal Pigment Epithelial Cells

Objective To study the relationship between the expression of sonic hedgehog signal pathway and that of vascular endothelial growth factor(VEGF)in hypoxia human retinal pigment epithelial(hRPE)cells.Methods hRPE-19 were divided into normal control group and hypoxia control g roup.And the cells were cultured for 4h,8h,12h,24h,then applicat the fluores cence quantitative PCR(rt-pcr)to detect the level of mRNA in the supernatan of Shh and VEGF;The concentrations of Shh and VEGF was measured by enzyme-li nked im-munosorbent assay in the content by using Pearson correlation analysis.Results The control group showed a small amount of Shh,VEGF mRNA and protein expression.The expression in the hypoxia experimental group was significantly higher than that in the control group,and the difference was statistically significant(F=178.364,P<0.01).Moreover,there was a statistically significant increase in the inhibition group of cyclopamine(F =68.745,P<0.01).In addition,the expression of VEGF protein in the hypoxia group was positively correlated with the expression of Shh protein(r= 0.942,P<0.05).And the expression of VEGF and Shh mRNA waspositively correlated(r= 0.970,P<0.01).However,The results showed that there was no significant correlation in hRPE cells of cyclopamine suppression group(r = 0.915,P>0.05).Conclusion Shh signal pathway is involved in the expression of VEGF in hRPE cells under hypoxia.