Expression Of Sirtuins Family In Mouse Kidney Is Chemia-reperfusion Model

Objectives:Renal ischemia reperfusion injury(RIRI)is a serious clinical disease,which has attracted people's attention because of its high prevalence and mortality.The Sirtuins family,an in vivo acetylase,is closely related to autophagy and is involved in ischemia-reperfusion of various organs.In this study,we established a mouse kidney ischemia-reperfusion model to clarify the expression of the Sirtuins family in this model,and provide a theoretical basis for studying the pathogenesis of renal ischemia-reperfusion injury,and provide a new target for the treatment of this disease.Methods:Six-week-old wild-type C57 mice were selected and divided into control group(Sham)and ischemic perfusion injury group(IRI),with 7 rats in each group(n=7).The IRI group used a kidney to block blood supply and establish a model of mouse kidney ischemia and hypoxia.The mice in the Sham group did not do any treatment after finding the kidneys.The blood supply was restored after 45 minutes in the IRI group,and the changes in color of the kidneys of the two groups were observed.After 24 hours,mouse serum was taken to detect creatinine and urea nitrogen levels,and renal function changes in the two groups were evaluated.The mice were sacrificed and the kidney tissues were taken.The protein expression level of Sirtuins family Sirt1-7 in mouse kidney tissue was determined by Western blot.The expression of m RNA in renal tissues of the two groups was detected by Real-time RT-PCR.In this experiment,the data were obtained by mean plus or minus standard deviation,and t-test was used for comparison between groups.P<0.05 was statistically significant.Results:1.The concentration of SCr in the serum of mice in the SCr:IRI group was 130.94±12.25 umol/L and the concentration of serum SCr in the Sham group was62.62±17.38 umol/L.The serum SCr concentration in the IRI group wassignificantly higher than that in the Sham group(P<0.05).2.Mouse BUN: The concentration of BUN in the serum of the IRI group was 14.70±1.63umol/L and the concentration of serum BUN in the Sham group was 14.58.62±2.26umol/L.The serum BUN concentration in the IRI group was not significantly higher than that in the Sham group(P>0.05).3.The m RNA expression of each member of n renal ischemia-r injuryin mice.We found that sirt1-4,6,7 showed different degrees of down-regulation in this mouse model,and the changes were significant in sirt1-3,6,7,statistically significant(P<0.05),while the gene expression of sirt5 was Elevated.Sirt1(IRI: 0.78 ± 0.15,Sham: 0.98 ± 0.12,P < 0.05),Sirt2(IRI: 0.69 ± 0.13,Sham: 0.97 ± 0.15,P < 0.05),Sirt3(IRI: 0.68 ± 0.15,Sham: 0.97 ± 0.12,P<0.05),Sirt4(IRI: 0.92±0.17,Sham:0.96±0.13,P>0.05),Sirt5(IRI: 0.99±0.13,Sham: 0.95±0.11,P>0.05),Sirt6(IRI: 0.75)± 0.14,Sham: 0.94 ± 0.16,P < 0.05),Sirt7(IRI: 0.65 ± 0.21,Sham: 0.93 ± 0.20,P <0.05).4.The expression of Sirt-related proteins in renal ischemia-reperfusion in mice,the expression of SIRT2,3,4,6,7 was down-regulated to varying degrees,Sirt4 was not statistically significant,and the rest were statistically significant(P <0.05).Sirt1,5expression was up-regulated(P<0.05).Sirt1(IRI: 3.81±0.15,Sham: 1.02±0.13,P<0.05),Sirt2(IRI: 0.69±0.13,Sham: 0.98±0.17,P<0.05),Sirt3(IRI: 0.10±0.08,Sham: 0.99± 0.11,P<0.05),Sirt4(IRI: 0.93±0.19,Sham: 0.95±0.13,P>0.05),Sirt5(IRI: 1.45±0.13,Sham: 1.05±0.12,P<0.05),Sirt6(IRI: 0.08)±0.03,Sham: 1.03 ±0.32,P < 0.05),Sirt7(IRI: 0.15 ± 0.12,Sham: 0.93 ± 0.20,P < 0.05).Conclusions:1.The expression of 2Sirtuins family genes and proteins is related to the occurrence of renal ischemia-reperfusion in mice;2.sirt2,3,6,7 genes and proteins were down-regulated in renal ischemia-reperfusion.We considered that this gene has a protective effect in renal ischemia-reperfusion,but its specific mechanism is still unclear.Need to be further explored.