MiR-3664-5P Suppresses The Proliferation And Metastasis Of Gastric Cancer By Attenuating NF-?B Pathway Via Targeting MTDH

Background:Gastric cancer(GC)is one of the most common and fatal cancers worldwide and the specific mechanism remains partially unknown.MiR-3664-5P was a rarely studied microRNA(miRNA),and this study aimed to figure out the relation of miR-3664-5P and GC.Methods:The expression difference of miR-3664-5P in GC and paired normal tissues was firstly detected with real-time PCR.EDU assay,CCK-8,Transwell assay and Flow Cytometry(FCM)assay were performed in vitro,the results of which were also verified with mice xenotransplantation model and lung metastasis model in vivo.Results:MiR-3664-5P was significantly downregulated in GC tissues and positively correlated with prognosis.Overexpression of miR-3664-5P suppressed,whereas inhibition of miR-3664-5P promoted the proliferation and metastasis of GC cells both in vitro and in vivo.After applying bioinformatic algorithms and luciferase reporter assay,MTDH was confirmed as the target gene of miR-3664-5P.MiR-3664-5P reduced the expression of MTDH and consequently downregulated the NF-?B pathway.Rescue experiments showed that suppression of MTDH restored the influence of miR-3664-5P inhibitors on GC cell line.Conclusion:Our research validated that miR-3664-5P suppressed the proliferation and metastasis of GC cells by attenuating NF-?B pathway via targeting MTDH,suggesting its potential to be an independent prognostic factor and biomarker in GC.