Study On Coaxial Printing Of Tissue Engineering Scaffold With Vessel-like Structure Based On Double Crosslinked Network

The loss or dysfunction of important vital organs is an important issue for human health.Traditional treatment methods can alleviate the damage but it is difficult to achieve complete repair of the organs.Organ transplantation is an effective way to improve this problem,but organ transplant donor resources are limited,expensive and risk of rejection.The construction of organoids in tissue engineering opens up new avenues for the treatment of such diseases,and the construction of vascular-like structures is the key to the regeneration of complex organs in tissue engineering.3D printing technology is a rapid prototyping technology widely used in tissue engineering.The use of 3D printing technology to construct vascular structures has become a hot research topic.In this paper,a bio-ink composed of sodium alginate and silk fibroin is used to print a highly permeable perfusion vascular-like structure,through a coaxial biological 3D printing system.The dysfunction or loss of vital organs is one of the major hazards faced by human health.Traditional treatments can alleviate damage but are difficult to achieve thorough repair.Organ transplantation is an effective way to improve this problem,but organ transplant donor resources are limited,expensive and risk of rejection.The construction of organoids in tissue engineering opens up new avenues for the treatment of such diseases.The construction of vascular-like structures in tissue engineering is the key to the regeneration of complex organs.3D printing is a rapid prototyping technology widely used in tissue engineering,the use of 3D printing technology to construct vascular structures has become a hot topic.In this paper,the coaxial printing system built by 3D bio-printer and coaxial extrusion system uses bio-ink composed of sodium alginate and silk fibroin to print a highly permeable perfusion-like vascular structure.Firstly,a brief overview of the research progress and development direction of key technology bio-three-dimensional printing of tissue engineering is given.The gel inks commonly used in bio-printing technology are enumerated and the existing gel channel manufacturing methods are summarized,so it laid the technical and material foundation for the method of coaxial bio 3D printing channels.The software and hardware components and methods of the 3D bioprinter and coaxial extrusion system syringe pump are introduced.According to the printing system and the characteristics of the hydrogel material itself,sodium alginate and silk fibroin were selected as biomaterials for constructing vascular-like scaffolds.Through the study of the rheological properties of the materials and the printing parameters,the following conclusions are drawn:Compared with pure sodium alginate,the silk fibroin-added composite gel has obvious shear thinning...