Experimental Research On Construction Of Small Diameter Vascular Graft Using Tissue Engineering Method

Part one Culture of seeding cells for vascular tissue engineeringObjective: To provide seeding cells for vascular tissue engineering research. Methods: Rabbits bone marrow was aspirated from the iliac crest sterilely with heparin. Rats bone marrow was obtained from femurs. Bone marrow mononuclear cells were isolated by gradient density centrifugation with lymphocyte separation medium. Mesenchymal stem cells (MSCs) were then obtained and cultured in L-DMEM. The growth, purification and proliferative abilities of MSCs were observed and their...