| Objective:To investigate the effects of vitamin D3 on children's pulmonary function,recurrence interval of asthma relapse and serum Toll-like receptor 4(toll-like receptors4,TLR4),and biochemical markers s100? in children with severe asthma adjuvant therapy.Methods: Selected 96 cases of children with severe asthma,according to the principle of randomly assigned into two groups,conventional treatment group and experimental group respectively,and the conventional treatment group given systemic corticosteroids,airway analysis and other routine drug treatment,the experimental group on the basis of routine therapy treatment,for nine consecutive days to give children with vitamin D3 oral intake treatment,dose according to oral 800 IU/day admission and hospitalization day 9,respectively,two groups of children with lung function measurements including the forced expiratory volume in 1 second(FEV1)and forced expiratory flow in exhaled50% lung capacity when the moment(FEF50 %),forced expiratory volume in 1 second(FEV1 / FVC),forced vital capacity ratio of forced expiratory flow in exhaled 25% lung capacity when the moment(FEF25 %),average after repeated measurements 3 times as the recorded date At the same time,TLR4 was detected by flow cytometry,and the serum S100 times was detected by ELISA,and the serum levels of TLR4 and S100 times were compared between the two groups.All the children were followed up for 1year,and the time of recurrence of asthma was recorded.Results: 1.There was no statistical difference in gender ratio,age and course of disease between the experimental group and the conventional treatment group at admission(P>0.05).Before and after treatment,there were no statistically significant differences in the mean disappearance time of cough,wheezing,chest tightness,wheezing in the lungs and shortness of breath between the two groups(P>0.05).2.No statistically significant difference was found in the lung function test indexes between the experimental group and the conventional treatment group at admission(P>0.05).After 9 days of treatment,the lung function indicators of the experimental group were PEV1=1.78 + 0.38 L,FEV1/FVC=63.10 + 4.41%,PEF25% =63.10 +11.01L/S,PEF50% =79.97 + 15.53L/S,and the lung function indicators of the experimental group were PEV1=2.39 + 0.40 L,FEV1/FVC=74.29 + 5.52%,PEF25%=73.39 + 11.75L/S,PEF50% =81.26 + 12.73L/S,both of the two groups showed significant improvement in pulmonary function,and both of the two groups showed statistically significant differences in pulmonary function after treatment and before treatment(P<0.05).After treatment,the pulmonary function indexes of the experimental group were statistically significant compared with those of the conventional treatment group(P<0.05).3.The mean value of serum TLR4 fluorescence was 44.6 ±8.4 ng/ml in the routine treatment group was 42.5 ± 7.8 ng/ml in the experimental group,and there was no significant difference in serum TLR4 expression between the two groups(P>0.05).The mean value of serum fluorescence was 21.2 ±10.2 ng/ml,18.8±6.9 ng/ml,and the expression level of TLR4 was decreased in the two groups compared with that before treatment,and the expression level of TLR4 was decreased in the two groups after treatment,showing a statistical difference(P<0.05).4.Before treatment,the expression level of S100?in the conventional treatment group was 1.2±0.4ng/L,and that in the experimental group was 0.8±0.5 ng/L,with no significant difference between the two groups(P>0.05).After treatment,the expression level of S100 in the routine treatment group was 0.8 ±0.4ng/L,and 0.41 ±0.3ng/L in the experimental group.The expression level of S100 in the test group was significantly lower than that before treatment.However,after treatment,the expression level of S100 in the test group was lower than that of the conventional treatment group,showing a statistically significant difference between the two groups(P<0.05).5.Survival analysis by kaplan-meier method showed that the average recurrence interval of asthma of children in the conventional treatment group was 9 months,and the average recurrence interval of asthma of children in the experimental group was 11 months,and there was a statistical difference between the two groups(P<0.05).Conclusion: Conventional treatment combined with vitamin D3 adjuvant therapy can effectively improve the pulmonary function of children,reduce the levels of TLR4 and S100 dosage protein,reduce the inflammatory response caused by asthma,and extend the time interval of recurrence,suggesting that this treatment program may be a better treatment... |
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