Total Flavonoids Of Mistletoe Osteoporosis Intervention Efficacy Substances And Meridian Research

Objective:The establishment of retinoic acid,ovariectomized osteoporosis model,effect of total flavonoids of Herba taxilli on osteoporosis rats,Objective to investigate the role of TFHT in reinforcing liver and kidney,strengthening bones and muscles,and its relationship with meridian tropism in rats.Methods:1.Total Flavonoids of Herba taxilli?TFHT?on related substances effect on osteoporosis induced by retinoic acid in rats and effect:female SD rats were randomly divided into Normal control,model,positive and normal to the medicine group and total flavonoids of mistletoe is high,medium and low dose group,except the normal control group,other groups on the daily afternoon 1%carboxymethyl cellulose sodium?CMC-Na?retinoic acid suspension 70mg·kg^-1,at TFHT high,medium and low dose group were given TFHT(400mg·kg^-1),(200mg·kg^-1),low dose(100mg·kg^-1)1%CMC-Na suspension,positive control group was given Zhuanggu Granule 1%CMC-Na suspension(5g·kg^-1),normal control group and model group were given 1%1%CMC-Na suspension.After 14 days of continuous administration,tretinoin was stopped,every day in the afternoon,the rats in each group continued to give the corresponding drugs or 1%CMC-Na suspension for 14 days.One week before the end of the experiment,the normal administration group was given a TFHT1%CMC-Na suspension 400mg·Kg^-1.This medicine for twenty-ninth days Rat model administration group and normal treatment group with 18 rats in each group at different time point in the blood from the abdominal aorta,and were immediately collected on both sides of the femur,heart,liver,spleen,lung,kidney,stomach,small intestine,large intestine,brain,uterus and ovarian factor,the remaining 50 rats were given blood from the abdominal aorta after 1h,the both sides of the femur,ovary,uterus+spleen,the weight of each organ and the weight of the left femur weight were recorded and recorded.The bone density of the left femur was measured by dual energy X ray bone densitometer,and the dry weight of the femur was measured.Analysis of left femur,bone weight,bone dry weight/wet weight/rat weight,bone weight,bone dry weight/rat bone weight,bone wet weight/bone length,bone wet weight/bone diameter SPASS,uterine and ovarian index coefficient and spleen coefficient,the right femur pathological sections of bone tissue morphology change.Micro plate method to detect serum calcium(Ca²⁺),determination of alkaline phosphatase in serum trace ELISA method?ALP?and UV spectrophotometry,tartrate resistant acid phosphatase?TRAP?content,HPLC detection model to the tissue of rats in avicularin,rutin,quercitrin content,use DAS3.2.8 software to handle the data analysis of the pharmacokinetic parameters.2.Effects of TFHT on ovariectomized osteoporotic rats and related functional substances:female rats were randomly divided intoNormal control,sham operation,model,positive,normal administration,TFHT high,medium and low dose groups.In addition to the Normal control group and the normal administration group,the other groups were operated on both sides of the ovary,and the sham operation group only removed a little fat near the ovary.7 days later,the Normal control group and sham operation group were given distilled water,the positive group Xianlinggubao suspension 300mgmg·Kg^-1,THFT,the normal administration,high,medium and low dose group were given TFHT(400mg·Kg^-1),(200mg·Kg^-1),low dose(concentration 100mg·Kg^-1).One week before the end of the experiment,the normal administration group was given a TFHT(400mg.Kg^-1).The weight of the animals was recorded weekly.The temperature control of isolated squirrel feeding cage was 20²5?,the humidity was 50%⁶0%,and the rats in each group were free to drink and feed.After 12 weeks of administration,18 rats in the model group and the normal administration group were taken blood from the abdominal aorta at different time points,and immediately killed two femur,heart,liver,spleen,lung,kidney,stomach,large intestine,small intestine,brain,ovary,thymus,spleen and other tissues.The remaining 60 rats after administration of 1H blood from the abdominal aorta,the bilateral femoral,ovarian,weigh and record each organ weight and wet weight of left femur weight,with dual energy X ray absorptiometry measured the left femoral bone density,bone mineral content,bone wet weight,dry weight and ash determination heavy.SPASS software was used to analyze indexes such as left femur density,bone mineral content,backbone weight,bone ash weight,bone wet weight/bone length,bone wet weight/bone diameter,uterine coefficient,thymus coefficient and so on.The right femur was stained with HE to make pathological sections for bone histomorphology analysis.Microplate method was used to measure serum Ca²⁺and Microenzyme ALP.ELISA method was used to measure estradiol?E₂?,interleukin 6?IL-6?,osteoprotegerin?OPG?,nuclear factor kB receptor activating factor ligand?RANKL?and transforming growth factor?TGF-beta 1?.HPLC detection model administration group and normal to the tissue of rats in avicularin,rutin and quercitrin content,using the DAS3.2.8software with the data analysis of the pharmacokinetic parameters.Results:1.TFHT can significantly improve the osteoporosis induced by retinoic acid in rat model of left femur bone mineral density,bone weight,bone weight/rat weight,bone wet weight/rat weight,dry weight/wet weight,wet weight of bone bone/bone length,bone wet weight/bone diameter,uterine and ovarian coefficient and reduce the index of spleen coefficient so,from the right side of femur pathological section to observe the morphological changes of bone tissue,bone tissue microstructure of TFHT can effectively prevent the retinoic acid caused the change,and can increase the content of Ca²⁺in serum of rats,decreased ALP and TRAP levels.The results of the analysis of effective substances in the model group?high dose group?showed that the AUC0-t of quercetin in tissues were stomach>kidney>liver>smallintestine>heart>lung>largeintestine>spleen>brain>serum,durationinvivocomparisonresultsshowedthatquercitrininthe liver=kidney=heart=brain=smallintestine=stomach=large intestine=lung>spleen=serum.AUC_0-t-t avicularin in tissues were stomach>spleen>small intestine>lung>kidney>liver>serum,AUC_0-t-t avicularin in tissues were stomach>small intestine>spleen>lung>kidney>liver,avicularindurationinvivoresultsfor liver=kidney=spleen=lung.=stomach=small intestine.Only a small amount of the distribution of Rutin in liver,stomach and brain.2.TFHT can significantly improve bone mineral density,bone mineral content,bone mineral weight,bone ash weight,bone wet weight/bone length,bone wet weight/bone diameter,uterine coefficient,reduce thymus coefficient,and improve bone microstructure in osteoporotic model rats.The levels of Ca²⁺,E₂,OPG,RANKL and TGF-beta 1 were increased in the serum of rats,and the levels of ALP and IL-6 were reduced.Rats in vivo efficacy and material analysis showed that quercitrin in various tissues of small intestine AUC_0-t-t small intestine>stomach>large intestine>kidney>lung>liver>brain>spleen>heart>serum.The time of maintenance is liver=kidney=heart=lung=stomach=large intestine=small intestine>brain=spleen>serum.Inalltissues,AUC_0-tis stomach>intestine>kidney>colon>lung>liver>brain>spleen.Duration of maintenance is kidney=lung=stomach=large intestine>small intestine>liver>brain>spleen.Rutin in tissues of AUC_0-t-t is small intestine>stomach>kidney>large intestine.The maintenance time was the stomach=the small intestine>the kidney>the large intestine.In the normal administration group,the AUC_0-tofquercetinwasgastric>smallintestine>kidney>liver>large intestine>brain>lung>spleen>heart>serum.The maintenance time was liver=lung=stomach=small intestine>kidney>spleen>brain>large intestine.AUC_0-t-t is a type of stomach>small intestine>kidney>liver>lung>spleen>brain>large intestine.The duration of maintenance is liver=lung=stomach=small intestine>kidney>spleen>brain>large intestine.Rutin AUC_0-t-t is the small intestine>kidney>stomach>heart>brain.The results of maintenance time were:kidney>small intestine=heart>stomach=brain.Conclusion:TFHT can effectively improve the retinoic acid,ovariectomized rat model of osteoporosis,showing the effect of Tonifying the liver and kidney,strong bones and muscles,showed that the active site TFHT for tonifying the liver and kidney,strong bones.TFHT avicularin,quercitrin in Loranthaceae tonifying the liver and kidney,strong bones and muscles of the main components.In accordance with the"effect of homologous"point of view,avicularin,quercitrin and sugariness is closely related.The traditional understanding of avicularin and quercitrin in vivo distribution in rat kidney,liver and mistletoe were relatively consistent.