Quality Analysis Of Different Specifications Of Antler Pieces

Objective:To establish a quality evaluation method for sika deer antler slices with different specifications.Methods:Sika velvet slices of different specifications were obtained through market purchasing and enterprise providing two channels,and identified according to the Chinese Pharmacopoeia 2015 edition;the content of water,ash and extract in the slices was determined;the content of hypoxanthine in the slices was determined by HPLC and the methodological investigation was carried out;and the nucleosides in the slices of different specifications were studied by HPLC.Characteristic atlas,methodological investigation and principal component analysis were carried out.The effects of antler extract on the survival rate of HEK293 cells were determined by MTT method,and the changes of the death rate of HEK293 cells induced by cisplatin were determined by MTT and LDH methods.The effects of antler extract on the damage of HEK293 cells induced by CDDP were detected by MDA and GSH kits,respectively.Changes in cell death rate of HEK293 induced by cisplatin in the same specification of antler extracts.Results:1.80 batches of sika deer antler pieces with different specifications were collected,including 20 batches of wax,powder,gauze and bone pieces.2.TCL method was used to identify glycine in antler slices,and the results were positive.The results of colorimetric identification accorded with the provisions of Chinese Pharmacopoeia on antler slices.3.The determination results of water,water-soluble extracts and alcohol-soluble extracts of antler slices showed that wax slices>powder slices>gauze slices>bone slices,total ash and acid-insoluble ash contents showed that bone slices>gauze slices>powder slices>wax slices.4.A method for the determination of hypoxanthine by HPLC was established.The content of hypoxanthine decreased from wax to bone slices.5.A method for the determination of 5 nucleosides in pilose antler slices was established.The Aglient Eclipse Plus C18?250 mm×4.6 mm,5?m?column was used.The mobile phase was methanol-0.07%glacial acetic acid solution with a flow rate of1.0mL·min^-1.The detection wavelength was 254nm and the column temperature was28?.6.Six common peaks were identified in the characteristic map.The results of principal component analysis showed that the content of characteristic components gradually decreased from wax to bone.7.A model of cisplatin-induced HEK293 cell injury was established.The antler extract concentration was 0.25mg·mL^-11 to 1.0mg·mL^-1,which could promote the growth of HEK293 cells.The antler extract had protective effect on the cytotoxicity induced by cisplatin,and the protective effect was strongest at 0.25mg·mL^-1.After adding antler extract to the preconditioning,the content of MDA in cells decreased and the content of GSH increased.Different sizes of antler slices have protective effect on cisplatin-induced HEK293 cells,but the effect is not obvious.Wax slices are better than other sizes of antler slices.Conclusion:The chemical constituents of different sizes of antler slices are quite different,and there is an adoptive trend from wax slices to bone slices.