Study On The Mechanism Of The Treatment Of Ulcerative Colitis By XinKaiKuJiang Representative Prescriptions

Objective: The rats were fed with the method of XinKaiKuJiang representative prescriptions to extract the drug-containing serum,and the ulcerative colitis(UC)model was simulated by the method of lipopolysaccharide(LPS)damage to Caco-2 cells.The protein expression was detected by cell viability,immunofluorescence and Western blot,and the mechanism of the treatment of ulcerative colitis cells by the method of XinKaiKuJiang representative prescriptions was explored.Methods: Caco-2 cells were used as research objects,and lipopolysaccharide(LPS)was used to make a cellular inflammation model.Twenty WB rats were divided into: blank group,western medicine control group(ie,sulfasalazine group),Wumei Pills group,Banxia Xiexin Decoction group,Gancao Xiexin Decoction group.The normal group was given water and food,and no drugs were given.The other groups were given sulfasalazine,Wumei Pills,Banxia Xiexin Decoction,and Gancao Xiexin Decoction for 7 days.Fasting water and extracting serum.Caco-2 cells were modeled with lipopolysaccharide,cell viability was measured with CCK-8,optimal time and optimal lipopolysaccharide(LPS)concentration were selected,and drug-containing serum was administered at the same time.The cells were grouped into normal group,model group and western medicine control.Group(sulphate),Wumei Pills,Banxia Xiexin Decoction,Gancao Xiexin Decoction.1)Cell viability was measured by CCK-8.2)The expression of TLR4/NF-?Bp65 protein was detected by immunofluorescence.3)The expression of TLR4/NF-?Bp65/IKB protein was detected by Western blot.Results: 1)Cell viability: Compared with the normal group,the cell viability injury in the model group was reduced(p<0.01).Compared with the model group,the cell viability of the prescription and the control group(sulphonium sulfapyridine)was improved.The cell viability of the normal group was 100%.The cell viability value of the model group decreased to 77% after lipopolysaccharide(LPS)injury,the cell viability value of the control group was 88%,and the cell viability value of the Wumei Pill group was 85%.The cell viability value of the soup group was 92%,and the cell viability value of the Gancao Xiexin soup was 76%.The difference was statistically significant(p < 0.05).2)TLR4/NF-?Bp65/IKB protein expression: Compared with the normal group,the model group showed high expression of TLR4/NF-?Bp65/IKB protein(p<0.01).Compared with the model group,the expression of TLR4/NF-?Bp65/IKB protein in Wumei Pills Recipe,Banxia Xiexin Decoction,Gancao Xiexin Decoction and Control group(Sulmonazopyridine)decreased,the difference was statistically significant(p<0.05).3)Immunofluorescence TLR4/p65 protein expression: Compared with the normal group,the expression of TLR4 protein was significantly increased in the model group,and the expression of NF-?Bp65 protein was increased.