The Effect Of Colla Corii Asini On Gut Microbiota And Body Inflammation In Patients With Primary Hepatic Carcinoma Treated With Transarterial Chemoembolization

ObjectiveCompare the gut microbiota before and after transarterial chemoembolization in primary hepatic carcinoma patients,and illustrate the role of Colla corii asini in the treatment of disorders of gut microbiota.Analysis the inflammatory,blood cell counts and function of liver after transarterial chemoembolization,and to evaluate whether Colla corii asini have the effect of alleviate inflammatory reaction,myelosuppression and liver damage caused by transarterial chemoembolization.MethodsWe enrolled twenty-nine patients with primary hepatic carcinoma who underwent transarterial chemoembolization,and randomly divided into two groups.There are twenty patients in experimental group,and Colla corii asini were taken during transarterial chemoembolization.Nine cases of patients in control group were treatment with transarterial chemoembolization alone.Fresh feces were collected 1 day before and 5 days after transarterial chemoembolization,respectively.The bacterial DNA was extracted for the feces and then microbiological sequencing base on Illumina HiSeq 2500 platform.Bioinformatics analysis was performed on the sequencing results,and Student's t-test was performed on the species abundance of the experimental group and the control group before and after transarterial chemoembolization using Metastats software.Significant analysis between groups was conducted at the taxonomic level of the phylum,class,order,family,genus and species.Using the analysis of variance of repeated measurement design,compare the blood cell counst,alanine aminotransferase,aspartate aminotransferase,albumin,bilirubin before and after transarterial chemoembolization between experimental group and control group.ResultsThe dominant flora in the intestinal tract of patients with primary hepatic carcinoma is Bacteroides,Firmicutes and Proteobacteria.Compared with the before of transarterial chemoembolization,the experimental group reduced six colonies after treatment,and added one colony,the control group reduced fifteen colonies,and added six colonies.In the control group,the Alphaproteobacteria disappeared after transarterial chemoembolization?P=0.000999?,the abundance of Lachnospiraceae was significantly decreased after treatment?P=0.033?,and the uncultured-bacterium-f-Rhodospirillaceae in proteobacteria disappeared after the treatment?P=0.000999?.The abundance of uncultured-bacterium-g-Atopobium in actinobacteria was significantly increased after transarterial chemoembolization?P=0.035?.In the experimental group,there was no significant difference in the gut microbiota between the before and after transarterial chemoembolization in the phylum,class,order,family.But the abundance of Atopobium and uncultured-bacterium-g-Atopobium in actinobacteria was increased after it?P=0.022,P=0.02?.The abundance of Lacnospiraceae-UCG-010 genus,uncultured-bacterium-g-Lachnospiraceae-UCG-010and uncultured-bacterium-g-[Eubacterium]-ventriosm-group in Firmicutes were significantly reduced in after the treatment?P=0.046,P=0.025,P=0.049?.After transarterial chemoembolization,white blood cell count,neutrophil count and neutrophil granulocyte percentage were significantly increased,erythrocyte count,hemoglobin and platelet count were significantly decreased?P<0.05?.After treatment,total bilirubin,direct bilirubin levels and glycocholic acid levels were higher than before treatment,total protein,albumin and prealbumin were lower than before treatment?P<0.05?.There was no significant change in alanine aminotransferase,aspartate aminotransferase,indirect bilirubin and globulin before and after transarterial chemoembolization.The changes in experimental group before and after transarterial chemoembolization were no different from those in the control group(F _interaction>0.05).ConclusionsThe transarterial chemoembolization causes the gut microbiota dysbiosis in patients with primary hepatic carcinoma.After transarterial chemoembolization,the Alphaproteobacteriadisappeared,the abundance of Lachnospiraceae decreased,some strains in proteobacteria disappeared,and the abundance of some strains in actinobacteria increased.Colla corii asini can alleviate the gut microbiota dysbiosis caused by transarterial chemoembolization in patients with primary hepatic carcinoma.The transarterial chemoembolization resulted in the red blood cell decrease and thrombocytopenia and liver damage,and a significant inflammatory response occurred after it.Colla corii asini can not alleviate the inflammatory,hematopoietic damage and liver damage caused by transarterial chemoembolization.