The Main Pharmacodynamics Study Of Colla Corii Asini Hematinic Soft Capsule

In recent years, as the situation of anemia in our country is getting more serious, the need of hematinic has become increasingly urgent. Therefore, Chinese Traditional Medicine, which can produce the synergistic and magni fyed effect, is known as" shotgun". Colla corii asini, one kind of the traditional Chinese medicine ginseng and pilose antler, was called "nourishing treble " and known as the blood panacea. The main raw material of tested drugs in the present study is colla corii asini, which also includes Radix Rehmanniae Preparata, Rhizoma Atractylodis Macrocephalae, Codonopsis, astragalus and Chinese wolfberry, and the soft capsule has the unique merit advantages, such as easy to carry and take away. It is designed to be applicable to Qi and blood deficiency syndrome caused by various causes, eye blend and coughing. In this study, the preliminary discussion was carried out about the main pharmacodyna mics of colla corii asini hematinic soft capsule and whether it was equivalent to colla corii asini hematinic ointment (old dosage form).Objective:To discuss about the main pharmacodynamics of colla corii asini hematinic soft capsule and whether it is equivalent to colla corii asini hematinic ointment (old forms), so as to provide scientific basis for further research and development.Methods:1The research of replenishing Qi and blood of colla corii asini hematinic soft capsule:many models were replicated, the model of hemorrhagic anemia, hemolytic anemia and bone marrow inhibited the hematopoiesis, and the cold diarrhea type model of spleen deficiency was induced by rhubarb Observation was made about peripheral red blood cell (RBC), hemoglobin (Hb), hematocrit (HCT), white blood cell (WBC), platelet (PLT), serum erythropoietin (EPO) content, organ index, bone marrow nucleated cell count, serum D-xylose concentration, independent activity times and grasping force index.2The research of immune function:it was respectively adopted the mouse carbon particle clearance test, murine antibody forming test induced by chicken erythrocytes and delayed type hypersensitivity reaction (DTH) test induced by two nitro benzene (DNFB) to determine the reticuloendothelial system phagocytic rate (K),phagocytic index (A), hemolysin antibody level by OD, ear swelling, swelling rate and organ index to observe the colla corii asini hematinic soft capsule effect on immune function.3The effect of atmospheric hypoxia tolerance of mice under normal pressure:by detecting the mean survival time (min) and the average extension rate (%) and other indicators, the body of the hypoxia tolerance function of raw materials of colla corii asini hematinic soft capsule was observed.Results:1The research of replenishing Qi and blood of colla corii asini hematinic soft capsule on mice:(1) the results of hemorrhagic anemia test showed, colla corii asini hematinic soft capsule and colla corii asini hematinic ointment both had anti hemorrhagic anemia effect, its mechanism was relevant to activate production of EPO secretion,to promote the generation of red blood cells, Hb content; it can improved the hemorrhagic the general state of anemia, increased grip and prolong swimming time. The efficacy of colla corii asini hematinic soft capsulell.10g kg-1dose group matched to colla corii asini hematinic ointment.(2) the hemolytic anemia test results showed, colla corii asini hematinic soft capsulel and colla corii asini hematinic ointment both had anti hemolytic anemia, can significantly improved peripheral RBC, Hb, HCT levels, stimulated the generation of peripheral blood cell.improved the general status of hemolytic anemia mouse, inhibited the increase of spleen and liver weights and organ index. The efficacy of colla corii asini hematinic soft capsulel2.21g kg-1dose group matched to colla corii asini hematinic ointment.(3) model of bone marrow hematopoietic function inhibition on mice induced by cyclophosphamide, colla corii asini hematinic soft capsulel and colla corii asini hematinic ointment both can increased WBC, RBC and PLT count, increased Hb content, stimulated the proliferation and differentiation of bone marrow nucleated cell number (such as the increase of myeloid hematopoietic precursors cells about the early, late immature cells number). Also can stimulated the proliferation of bone marrow erythroid about young, late immature cells and megakaryocytic progenitor cells; made the weight of immune organs (Tthymus,Spleen organ) and organ index increase. The medicine can effectively improved blood deficiency symptoms, namely can produced antagonistic action on bone marrow hematopoietic inhibition induced cyclophosphamide in rats. The efficacy of colla corii asini hematinic soft capsule2.21g kg-1dose group matched to colla corii asini hematinic ointment.(4) the cold diarrhea type model of spleen deficiency induced by rhubarb, colla corii asini hematinic soft capsulel and colla corii asini hematinic ointment showed certain replenishing Qi and nvigorated spleen function, can increased food intake and body weight in mice with spleen deficiency syndrome, body temperature picked up, enhanced grip and prolong mice swimming time, increase the weight of the immune organs (thymus, spleen), liver and organ index. The efficacy of colla corii asini hematinic soft capsule2.21,1.10g kg-1dose group matched to colla corii asini hematinic ointment.2The research of immune function on mice (1) carbon clearance test, colla corii asini hematinic soft capsulel can improve the mouse’s peritoneal macrophage phagocytosis rate and phagocytosis index, showed that raw materials of colla corii asini hematinic soft capsulel can effectively enhance nonspecific immune function of mice. Wherein, The efficacy of colla corii asini hematinic soft capsule0.55g kg-1dose group match to colla corii asini hematinic ointment.(2) The murine antibody forming test induced by the chicken red blood cell, colla corii asini hematinic soft capsule can improve the level of humoral immunity on mice. Wherein, the effect of colla corii asini hematinic soft capsulel.10,0.28g kg-1dose group is obvious, and are stronger than colla corii asini hematinic ointment.(3) DTH induced by DNFB, colla corii asini hematinic soft capsulel and colla corii asini hematinic ointment can reduced swelling and swelling rate on male mice, and elevated on the female mice. The regulation of cellular immune function on male and female mice showed a bidirectional regulation effect, so make the level of male mouse immune cells down-regulation (inhibition), the female mice cellular immune upregulation (promotion). Wherein, for male mice, colla corii asini hematinic soft capsule0.55g.kg-1dose group inhibition is significantly; for the female mice, the stimulative effect of colla corii asini hematinic soft capsule2.21g. kg-1dose group is most pronounced.3Normal pressure hypoxia tolerance function on mice:bearing hypoxia at normal pressure experimental results show, colla corii asini hematinic soft capsule can significantly enhance the hypoxia tolerance on mice, the mice survival time, prolong the average rate, colla corii asini hematinic soft capsule1.10g kg-1dose showed stronger anti-stress effect than colla corii asini hematinic ointment.Conclusion:Colla corii asini hematinic soft capsule has replenished Qi and blood function, regulates immune function, enhances the body atmospheric hypoxia tolerance function, and has considerable effect comparing to the colla corii asini hematinic ointment.