Inhibition Of TMEM45A Suppresses Roliferation,induces Cell Cycle Arrest ?reduces Cell Invasion In Human Ovarian Cancer Cells

Objective: The effect of TMEM45 A gene on the proliferation,adhesion and invasion of ovarian cancer cell line was observed through the knockdown of TMEM45 A.The TMEM45 A gene was enriched and analyzed to find the signal pathway related to it.The aim of this study was to elucidate the role of TMEM45 A in the cell proliferation and invasion of ovarian cancer cell lines and to further explore its possible signaling pathways.Methods:We compared the expression of TMEM45 A between ovarian cancer and normal tissues based on the RNA-sequencing data of the ovarian cancer cohort from The Cancer Genome Atlas(TCGA)project and our data of real time PCR from 25 pairs of ovarian cancer tissues and the matched non-cancerous tissues.And inhibition the expression of TMEM45 A by RNA interference in ovarian cancer cell lines:HO-8910 and A2780;Cell proliferation?cell cycle distribution ?cell adhesion and invasion ability were detected by Cell Counting Kit-8 assay(CCK-8),propidium iodide(PI)staining,and cell adhesion and Transwell assays.In addition,the level of RNA and protein of transforming growth factor-?(TGF-?1 and TGF-?2),Ras homolog family member A(Rho A)and Rho-associated kinase 2(ROCK2)were detected by real time PCR and western blotting.Results:1?TMEM45A express overly in ovarian cancer;2?silence of TMEM45 A impressed cell proliferation obviously and significantly increased cell population in the G1 phase.3?Kockdown TMEM45 A inhibited cell adhesion and invasion.4?supperssion of TMEM45 A notably downregulation the expression of TGF-?1/2?Rho A and ROCK2?Conclusion:TMEM45A may work as a oncogene of ovarian caner,so inhibiton TMEM45 A may be a therapeutic strategy of ovrian cancer.