Autophagy And Associated Inhibition Of Raptor,Rictor Relationships With Angiogenesis And Significance During Of Colorectal Cancer

Objective:To detect the expression of Raptor,Rictor,angiogenesis-related factors HIF-la,HIF-2a and VEGF and investigate their relationship and significance in colorectal cancer(CRC).To evaluate the relationship and clinical significance between the expression of autophagy-related factors Raptor,Rictor and LC3,and the expression of angiogenesis-related factors HIF-1?,HIF-2? and VEGF in different stages and different parts colorectal cancer(CRC).Materials and MethodsA total of 120 CRC samples at different stages were collected from the Department of Pathology of Binzhou Medical University Hospital between January 2013 and June 2016.Samples included advanced colorectal cancer,early colorectal cancer,respectively,60(According to the location of the tumor is divided into the central area and the marginal area.).60 cases of normal colorectal mucosa were selected as control.(away from the tumor margin>5cm);Parts of the tissue were immediately frozen in liquid nitrogen,and other parts were routinely fixed in 10%buffered neutral formalin.All CRC patients were clinically and pathologically proven to have not received preoperative chemotherapy or radiotherapy before operations.All specimens were collected with the informed consent of the patients.The expressions of Raptor,Rictor,LC3,HIF-la,HIF-2a and VEGF in normal colorectal mucosa,early colorectal cancer,advancedcolorectal cancer were detected by immunohistochemistry,Western blot and real-time PCR.Toanalyzed the expression of Raptor,Rictor,LC3,HIF-la,HIF-2a,VEGF and clinicopathologicalparameters in different organizations.The expression of Raptor,Rictor,LC3,HIF-la,HIF-2a and VEGF in colorectal cancer were analyzed.ResultsThe expression of Raptor,Rictor,LC3,HIF-la,HIF-2a and VEGF in each group of CRCwere significantly higher than those in normal colorectal mucosa.The expression of Rictor,LC3 and HIF-2a in the center of advanced colorectal cancer were significantly higher than those in themargin of advanced colorectal cancer.And the expression of Raptor,HIF-la and VEGF in themargin of advanced colorectal cancer were significantly higher than those in the center ofadvanced colorectal cancer.But there was no significant difference between the margin of advanced colorectal cancer and early colorectal cancer.The MVD was higher in patients with Raptor or Rictor positive than that in patients Raptor or Rictor negative.The expression of Raptor is negatively correlated with LC3 and positively correlated with HIF-1? and VEGF in CRC.The expression of Rictor is positively correlated with LC3?HIF-2? and VEGF.Raptor?Rictor and LC3 expression in poorly differentiated CRC is higher than that in well differentiated CRC.The expression of Raptor,Rictor,LC3,HIF-1?,HIF-2? and VEGF in lymph node metastasis is higher than that obtained in the absence of lymph node metastasis.In addition,they is related to the depth of invasion of the tumor.Conclusion1.Raptor and Rictor are closely related to the angiogenesis of colorectal cancer,which can promote the angiogenesis of colorectal cancer by affecting HIF-1? and HIF-2?,respectively.2.The expression of Raptor,Rictor and LC3 in different colorectal cancer tissues were different,which was closely related to the occurrence and development of colorectal cancer.3.Different levels of autophagy in different periods of colorectal cancer and advanced cancer in different areas of the impact on angiogenesis and its significance,and through different ways to affect tumor angiogenesis.4.Autophagy-related factors Raptor,Rictor,LC3 are closely related to the development,differentiation and metastasis of colorectal cancer,which is an important factor influencing prognosis.