| Aim: Human hepatocellular carcinoma(HCC) is a common malignant tumor in digestive system, but its mechanism is still unclear. Rictor is a keycomponentof m TORC2, and recently, it was detected at high levels in the progression of lung cancer, breast cancer and prostatic cancer and so on. The tumor suppressor p53 acts as a transcription factor and regulates the expression of m RNA and micro RNA. In this research, we hope to work out the expression levels of Rictor in HCC, and the relationship between tumor suppressor p53 and Rictor.Methods: We measured expression of Rictor in 26 pairs of HCC samples and para-carcinima tissue by immunohistochemistry. Human Rictor was attenuated by Si RNA in Huh7 cell line and then identified the effect by Western blotting. The proliferation and migration of cells transfected with si RNA were detected by CCK-8 and Transwell, respectively. The recombinant plasmidexpressing wild-type p53 were constructed and transfected into Hep3 B, then we verified the expression levels of m RNA and protein of p53 and Rictor. The databases, such as Target Scan and mi RNAbase, were used to predict the mi RNAs which targeted Rictor and were induced by p53. And then, to find out that Rictor can be one target of this mi RNA, eligible mi RNA was up-regulated in Hep3 B by transfected with mi RNA mimic. Rictor expression was assessed by western blotting after mi RNA had been over expressed in Hep3 B.Result: Compared with normal tissue, Rictor was over expressed in HCC tissue. Western blotting demonstrated reduction of Rictor in Si RNA transfected Huh7 cells. Down regulation of Rictor impaired the Huh7 cells proliferation and migration. Wild-type p53 could be detected in Hep3 B cells which transfected with recombinant plasmidexpressing wild-type p53, and at the same time wild-type p53 down-regulated the protein expression of Rictor. The database showed that Rictor may be a predicted target of mi R-192, which was one of the mi RNAs induced by p53. And then in Hep3 B, over expression mi R-192 down-regulated the protein level of Rictor and p473-AKT.Conclusions: The expression of Rictor was up regulated in HCC tissue. Knocking down the expression of Rictor could weaken the ability of migration and proliferation in HCC cells. Wild type p53 may regulate Rictor expression by inducing mi R-192. |
