Animal Study Of Methylene Blue Fibrin Composite Gel As A Preoperative Localization Agent For Pulmonary Nodules

Objective:To explore the clinical value of methylene blue fibrin complex gel as a preoperative localization agent for pulmonary micronodules,and to provide a basis for accurate location of pulmonary micronodules before operation.Methods:1.Study the influence of methylene blue on fibrin gel dissolution rate.With fibrinogen,thrombin,calcium chloride,methylene blue material,production of methylene blue fibrin gel: fibrinogen concentration according to 10 mg / ml.And set up a control group without methylene blue.Each group of fibrin gels was placed in a constant temperature water bath at 37 ° C,the dissolution was observed every 24 hours and the dissolved volume was measured.2.Effect of fibrin gel on diffusion rate and absorption rate of methylene blue in lung tissue:Twenty-five healthy New Zealand rabbits weighing 2.0-2.5 kg were randomly divided into ?,?,?a,?b and ?c groups,with 5 in each group.New Zealand rabbits in group ? were injected with methylene blue thrombin(first)+ fibrinogen(after)(a total of 0.58 ml)in the right upper lobe,group ? New Zealand rabbits were injected fibrinogen(first)+ methylene blue thrombin complex(after)(total 0.58ml)in the right upper lobe,New Zealand rabbits in group ? were injected with methylene blue(0.58 ml)in the right upper lobe.Puncture operation method to take the No.6 needle in the New Zealand rabbit subclavian midline positioning puncture depth of 1.0 ~ 1.5cm,after withdrawing the gas were injected methylene blue fibrin gel complex or methylene blue.On the 1st,2nd,3rd,4th and 5th days after injecting the methylene blue fibrin compound gel,one of the New Zealand rabbits of Group ? and ? were sacrificed respectively;in the first injection of methylene blue 1,2,3 hours were killed ? a,? b,? c New Zealand white rabbit five,obtain lung pathology specimens,HE staining,microscopic observation of pathological changes [1].Results: 1.During the whole preparation of the gel,the time required for forming the fibrin gel in each group was between 40-60 s,the addition of methylene blue did not affect the gel formation time,and eventually both formed a jelly-like solid.2.New Zealand rabbits in group?,? were injected with methylene blue fibrin gel mixture after percutaneous puncture on day 1,2,3,4,5,respectively,two groups of New Zealand rabbits each anatomy,take the lung specimens visible injection Of the lung a blue jelly-like jelly-like substance,with the passage of time,the diameter gradually decreased until the 5th day disappeared.Group ? New Zealand rabbits percutaneous puncture after injection of methylene blue 1,2,3 hours were killed ?a,? b,?c group of New Zealand rabbits each five,when taking lung specimens,?a,? b group New Zealand rabbit eye visible injection of lung tissue was stained with methylene blue,?c group lung tissue no obvious color.Conclusion:1.Methylene blue does not affect the release rate of fibrin gel release system;2.The subject to fibrin gel as a carrier,methylene blue as a reagent in the New Zealand rabbit lung tissue successfully established a blue class round nodules model,with the passage of time,the nodules continue to change Small until disappeared on the fifth day,consistent with in vitro release of methylene blue fibrin gel system.