Effect Of SB525334 Combined Batimastat On The Invasion And Growth Of Glioma U87 Cell

Objective:At this stage,it is known that the treatment and rehabilitation of cranial gliomas has always been one of the major problems that plague neurosurgeons,especially glioblastomas(WHO classification is class IV),which develops rapidly and is normal to the surrounding.The strong invasive ability of the brain tissue and the poor patient characteristics are also one of the difficulties that hinder the further development of neurosurgery.Even patients who received a widely accepted treatment plan,surgery+radiotherapy+chemotherapy,are more difficult to satisfy.Therefore,understanding the development mechanism and its influencing factors of gliomas in depth and finding the breakthrough to cure the disease are the primary problems at present.Studies have shown that glioma itself secretes matrix metalloproteinase-2 and matrix metalloproteinase-9,and its main function is to degrade extracellular matrix and achieve invasion.Transforming growth factor-?1 and prostaglandin E2 can increase the expression of matrix metalloproteinases through different pathways.In this study,through comparative studies,the combined effect of transforming growth factor-?1inhibitor and prostaglandin E2 receptor antagonist blocked both of them.The pathway of action reduces the expression of matrix metalloproteinases and thus more effectively reduces cell invasiveness.Methods:1.MMT assay to detect changes in cell viability;2.Transwell assay to detect cell invasion viability;3.Gelatin zymography was used to detect the viability of activity of MMP-2 and MMP-9;4.Statistical analysis: SPSS23.0 statistical software was used to analyze the data.The data of the measurement type,if obeying the normal distribution,are expressed as the mean and the standard deviation;the comparison between the three groups and three or more sets of measurement data is performed using the one-way ANOVA,and the multiple comparisons are performed using the LSD.If the data of the measurement type does not obey the normal distribution,the median and interquartile range are used,and the rank sum test is used for the comparison between groups.The test level ? was 0.05,p<0.05,statistically significant.Results:1 The survival rate of U87 cells was measured by MTT assay.The cell viability(70.648±13.334)% in the SB525334+Batimastat group was lower than that in the Batimastat group(85.069±91.858)%;and it was less than in the SB525334 group(78.290±12.979)%.2 SB525334 and Batimastat can reduce the activity of MMP-2 and MMP-9.The expression of MMP-9 in the transforming growth factor inhibitor + prostanoid receptor antagonist group was lower than that in the control group(1936.897±101.271)(2613.118±155.694);the transforming growth factor inhibitor + prostanoid receptor antagonist group MMP-9(1936.897±101.271)was less than MMP-9 in the transforming growth factor inhibitor group(2413.118±155.694);MMP-9(1936.897±101.271)in the transforming growth factor inhibitor + prostanoid receptor antagonist group was less than MMP-9 in prostanoid receptor antagonist group(2052.024±171.484).The expression of MMP-2(523.992±55.629)in the transforming growth factor inhibitor + prostanoid receptor antagonist group was lower than that of the control group(629.792±46.289);the transforming growth factor inhibitor + prostanoid receptor antagonist group MMP-2(523.992±55.629)was less than MMP-2 in the transforming growth factor inhibitor group(584.361±46.680);MMP-2(523.992±55.629)in the transforming growth factor inhibitor+prostaglandin receptor antagonist group was less than MMP-2 in prostanoid receptor antagonist group(554.388±55.988).3 SB525334 and Batimastat can reduce the invasive ability of U87 cells.The number of migrating cells in the SB525334 group(92.083±4.853),the number of migrating cells in the Batimastat group(80.262±6.461),and the number of migrating cells in the SB525334+Batimastat group(55.180±6.375)were less than those in the control group(119.680±9.134);The number of migrating cells in the Batimastat group(80.262±6.461)and the number of migrating cells in the SB525334+Batimastat group(55.180±6.375)were lower than those in the SB525334 group(92.083±4.853);the number of migrating cells in the SB525334+Batimastat group(55.180±6.375).The number of migrating cells was less than that of Batimastat group(80.262±6.461).Conclusion:1.SB525334 and Batimastat can reduce the survival rate and proliferation of U87 cells and can effectively reduce the invasion of U87 cells.2.SB525334 and Batimastat can reduce matrix metalloproteinase activity.