The Preliminary Identification Of The Disease Of Lophomonas Blattarum

ObjectiveSince the first case of lophomonas blattarum is reported,more and more cases emerged,even a literature reported 24 cases of L.blattarum.At present,the diagnosis method of L.blattarum mainly for microscopic morphological diagnosis.The auxiliary diagnosis is that eosinophils increased and the treatment by metronidazole is effective.However,human ciliated cells are similar to L.blattarum under ordinary optical microscope,and asthma and other diseases can be accompanied by increased eosinophils.Metronidazole is also effective for anaerobic infection.Therefore,the reliability of the method in the diagnosis of L.blattarum and authenticity of the existence of the disease become great controversy.The misdiagnosis of the disease will mislead the clinical treatment,and cause the delay of the patient's condition and the waste of medical resources.At the same time,the story of the L.blattarum to infect the human body has been reported by a lot of newspapers,causing public panic.Hence,we urgently need better diagnostic methods and more real conclusions to end the current dispute and provide a reliable direction for clinical treatment.In order to end the current dispute of diagnosis and treatment,this article is to start with two aspects of case analysis and molecular diagnosis.This study did not interfere with clinical treatment,just tracing the treatment process and the treatment results of patients.At the same time,This study got the worms which are similar with L.blattarum in the predecessors' articles from the hindgut of cockroach.And we use the application of degenerate primers of the housekeeping gene GAPDH of Cristamonadida,and amplify the sequence of GAPDH by RT-PCR.Method(1)Observe the alveolar lavage fluid under ordinary optical microscope.If the "L.blattarum" was found,we would record the patient's ID and trace their condition.(2)Dissecte the cockroach(Periplaneta americana)posterior intestinal tract and obtain the living bodies,which is highly similar with L.blattarum in appearance to the previous articles.(3)Synthesize the GAPDH gene primers of the genus Lantana in the citations.(4)Established the detection system of the genome of L.blattarum with RT-PCR technology as the core and evaluate its detection performance.(5)Use the RT-PCR detection system to detect suspected clinical specimens.ResultWithout interfering with clinical treatment,156 cases of Blattella trichomoniasis in BALF were found to be cured by antibiotics and physical therapy.(2)The primers of the Trichomonas vaginalis were proved to be available.(3)Evaluation of the detection performance of RT-PCR:(1)There is no cross reaction with bacteria,fungi and human genome DNA in the sputum of human body.(2)Mixed L.blattarum into0.5ml's insect-free sputum,and the minimum detection limit that could be reached is5 insect bodies.(4)The clinical specimens with "L.blattarum" observed under ordinary light microscope are tested by RT-PCR,and 156 groups of "L.blattarum" were negative.Also,the cells are more than 5.conclusionCiliated cells are easily misdiagnosed as L.blattarum,because the appearance of them are similar and the environment of sputum and alveolar lavage is complex.Case analysis and the experimental results also show that lophomonas trichomoniasis reported by a large number of literature is very questionable.