Comparative Study On Intervention Effects Of Different Therapeutic Methods Based On Drug Pair On Lewis Lung Cancer

Objective:.Four groups of drugs were selected:White Snake-tongue grass-half lotus,Banxia-poria,peach kernel-Angelica sinensis,Astragalus membranaceus,respectively,on behalf of Qingre jiedu method,Phlegm Dispel dampness method,Huoxue Huayu method,Fuzheng consolidation method,through the comparison of four groups of drugs to represent the different treatment of Lewis lung cancer tumor-bearing mice lung adenocarcinoma cell intervention effect,The mechanism of intervention effect was preliminarily discussed from the aspects of tumor immunosuppression microenvironment,immunity and inflammation level,and tumor metastasis,which provided a preliminary theoretical and experimental basis for rational and effective application of the corresponding Chinese medicine in the treatment of lung adenocarcinoma.Method:1.Establishment of a tumor-bearing model of C57BL/6 mice:subcutaneous injection of 1ml insulin syringe on the left upper limb of the mouse under the armpit skin of 0.2ml Lewis lung cancer cell suspension(concentration of 1.5x107).To observe the proliferation of tumor cells,it was shown that the model was successful when the mouse skin touched mung bean size tumor.The effectiveness of tissue morphological evaluation model was observed by pathological section.2.Comparison of intervention effects of different therapeutic methods based on drug pairs on Lewis lung cancer:The 36 mice were randomly divided into 6 groups:Model group,Positive group,Qingre jiedu Group,dispelling phlegm and dampness group,activating blood Stasis group,reinforcing and consolidating group,and 6 in each group.Select 6 Normal Mice as a blank group.From the day of the success of the model to the No.0 day(D0),from the 1th Day(D1),the continuous medicine 15days.Daily observation of mouse mental state,activity,hair gloss and supple,the condition of defecation,etc.D1 weighing,from the D2,the other day to take the weight of mice,to draw the weight change curve;From the D5,from the time of 2nd,with vernier caliper to measure the longest diameter A and the shortest path B,the tumor volume=1/2(ab~2)indicated the tumor size,Draw the growth curve.In the D16,the tumor weight was calculated,the tumor-suppressor rate was computed,the tumor-suppressor rate(%)=(average tumor quality of the model group-the average tumor quality of the intervention Group)/The average tumor quality of the model group was x100%.The tissue pathology(HE staining)of the tissue was observed.3.The related mechanism of different treatment methods based on drug pair on Lewis lung cancer:the 16th day after the animal was called quality,the orbital blood was detected under sterile condition,and the concentration of IL-10 and tnf-a in serum was examined by Elisa method.After the tumor is weighed,take part of the tumor system for paraffin section,The expression of CD68 positive tams in tumor tissues was detected by immunohistochemistry.The expression of E-cadherin and vimentin in tumor tissues was detected by Western blot method.Results:1.The molding success rate is 100%.The tumor is located in the left forearm of the mouse,with the hand can touch the nodules of the size of the green bean,the texture is hard,the boundary of the tumor is clear,the shape rule,the activity degree is poor.It was observed that in situ tumor was fish-colored and tough.Tumor tissue HE staining can be found in the tumor.2.The tissue HE staining can be found in the tumor;Groups can improve a tumor-burdened survival in mice,reducing tumor cell nucleus division,reducing tumor growth trend,inhibiting tumor growth volume,each inhibitory rate is:positive group respectively(53.20%),heat-clearing and detoxifying(47.29%),activating blood and removing stasis group(43.35%),phlegm clearing damp group(36.45%),the supporting the healthy energy group(18.23%).3.After the drug intervention,compared with the model group,the content of CD68 positive TAMs in the positive group and the active blood stasis group was lower-a significant difference(P<0.05).Compared with the positive group,the content of CD68 positive TAMs in the detoxification group,the model group and the fuzheng group was higher,and the difference was statistically significant(P<0.05).The content of CD68 positive TAMs was higher than that in the blood circulation group,and the difference was statistically significant(P<0.05).The concentrations of IL-10 in the positive group and the detoxification group were lower than that in the model group,and the levels of il-10 in the group of the clearing damp group,the strengthening group and the blood circulation were higher than that in the model group.Among them,the level of il-10 was the highest in the blood circulation,which was significantly different from that of the other groups(P<0.05).The il-10content in the fuzheng group was higher than that in the model group,the positive group and the detoxification group,and the difference was statistically significant(P<0.05).Compared with model group,the rest of the groups of TNF-a levels are low,among them,the phlegm and clearing damp group concentration of TNF-a minimum,and the blank group,model group,positive,promoting blood circulation to remove blood stasis group,with significant difference(P<0.05);The concentration of TNF-a in the detoxification group was slightly higher than that in the clearing damp group,but it was lower than that in the blank group,model group and positive group,and the difference was statistically significant(P<0.05).The TNF-a concentration was lower than the model group,and the difference was statistically significant(P<0.05).The concentration of TNF-a in the detoxification group was lower than that in the blood circulation group,and the difference was statistically significant(P<0.05).The relative quantitative results of the wave protein were ranked as(from high to low):fuzheng fixed group>blank group>heat-clearing and detoxifying group>activating blood stasis group>phlegm clearing damp group>positive group>model group;There was a significant difference between the fuzheng group and the sputum clearing damp group,the positive group and the model group(P<0.05).There was significant difference between the detoxification group and the model group(P<0.05).There was significant difference between the model group and the blank group(P<0.05).E-calcium adherent protein was not detected.Conclusion:1.The different treatments have a certain benign intervention effect on tumor growth of Lewis lung cancer tumor,which can inhibit the growth of Lewis lung cancer.It is the best method for the elimination of heat and detoxification by heat and detoxification method.2.According to the different medicine for treatment can improve the tumor immunosuppression microenvironment,adjust a tumor-burdened immune suppression or immune stimulation reaction,reduce a tumor-burdened body inflammatory reaction,strengthen anchor connection between cells,inhibit cell free fall off transfer to tumor suppression effect into full play.