Establishment Of P-phenylene Diamine Test Method And Investigation Of Occupational Health Situation

Objective:To establish a method for the detection of p-phenylenediamine in the air of workplace and test its performance.Based on the established method for testing p-phenylenediamine,field investigations and laboratory tests of p-phenylenediamine in the air at the workplace were conducted to assess the occupational health status of p-phenylenediamine in the workplace.Combined with physical examination and the questionnaire survey of autonomic status,we comprehensively evaluated the effect which exposed to p-phenylenediamine in the workplace on the physical and mental health of workers.In summary,it provides the basis for the detection of p-phenylenediamine and the establishment of occupational exposure limits in the air of the workplace.Method:1?The high performance liquid chromatography system with UV detector was selected to determine the retention time of p-phenylenediamine and draw the standard curve which with the reference to the test method of p-phenylenediamine in the air of workplace in foreign countries,and based on the existing research in our country.At the same time,we must detect the each performance index of the testing method to establish the laboratory testing methods for p-phenylenediamine in the air of the workplace.2?Using fixed-point sampling and individual sampling to survey the p-phenylenediaminea of hair dye company to determine the concentration of p-phenylenediamine in the air of the workplace and occupational health conditions.A cross-sectional study was conducted on 54 workers in the enterprise,including 38p-phenylenediamine contact workers and 16 control workers.Physical examination(including blood pressure and pulse rate,blood routine,liver function and pulmonary function)and questionnaires of the selected subjects were conducted.The questionnaire mainly consisted of three scales:SF-36,DLQI and VAS.Result:1?Under the selected chromatographic conditions,the linearity of p-phenylenediamine in the range of 0.07-10.00?g/mL was good with the correlation coefficient R~2>0.9990,the detection limit 0.02?g/mL and the limit of quantification0.07?g/mL.The sampling volume was 45 L,the lowest detected concentration was0.00089 mg/m~3,the lowest quantitative concentration was 0.0031 mg/m~3,the relative standard deviation was 1.40~2.60%,the average sampling efficiency was 96.6%and the elution efficiency range was 91.4~95.4%.Glass fiber membrane was used to collect p-phenylenediamine in the air of the workplace.Samples were stored at room temperature for 7 days.2?Occupational sites test results showed that in fixed-point sampling,the concentration of p-phenylenediamine was 0.01~0.19 mg/m~3 at the ingredient operating site,0.0038~0.12 mg/m~3 at the feeding operation site,0.00089~0.04 mg/m~3at the batching operator and<0.00089 mg/m~3 at packing operation site.In individual sampling,the concentration of p-phenylenediamine was 0.025~0.047 mg/m~3 at the ingredient operating site,0.0003~0.015 mg/m~3 at the feeding operation site,0.0003~0.03 mg/m~3 at the batching operator and<0.00033 mg/m~3 at packing operation site.3?Health monitoring results of the subjects showed that:the physical examination are in the normal range,no obvious abnormalities in the p-phenylenediamine exposure group and the control group.The general situation,blood routine,liver function,renal function and other data in the contact group and the control group were tested by two independent samples t-test found no significant difference between the two groups(P>0.05).4?Questionnaire results of autonomic status of the study subjects showed that scores of 9 aspects of the SF-36 scale of the contact group and the control group were respectively tested by two independent samples t-test,and the score of energy,emotional function and general health status in the contact group and the control group were statistically significant,and the control group in these three aspects was significantly better than the exposure group(P<0.05).There was no significant difference between the two groups in the remaining six aspects(P>0.05).The scores of the DLQI scales of the contact group and control group were respectively tested by the rank sum test of two independent samples,and the results showed that there was no significant difference between between these two groups(P<0.05);The scores of the VAS scale of the contact group and control group were respectively tested by the rank sum test of two independent samples,and the results showed that there was a significant difference between these two groups(P>0.05)Conclusion:1?P-phenylenediamine solution elution-high performance liquid chromatography had a high sensitivity and good peak shape,and the method of performance indicators and sampling efficiency tests are conformed to our GBZ/T210.4-2008"Occupational Health Standards Development Guide Part 4:Determination of workplace air chemicals approach"which applies for the detection of the concentration of p-phenylenediamine in the workplace air.2?The high concentration of p-phenylenediamine at the ingredient operating site and the feeding operation site has exceed the limit of occupational exposure to p-phenylenediamine in the air of workplace(0.1 mg/m~3)in the United States.The concentration of p-phenylenediamine in the individual sample did not exceed the exposure limit.3?The physical examination indicators of p-phenylenediamine exposure group and control group were normal.4?There have some effect on people's quality of life and subjective feelings in the contact group throught exposed p-phenylenediamine.Although there is no obvious dermatological problem in the contact group,there is an uncomfortable condition in the skin.