The Tropism And Mechanism Of Human Umbilical Cord Blood Mesenchymal Stem Cellsin Human Tongue Squamous Cell Carcinoma

Objective:The oral cancer accounts for more than half are tongue squamous cell carcinoma,and early local infiltration and lymph node metastasis are hallmarks of squamous cell carcinoma of the tongue,which usually leads to failure of chewing,speech and swallowing difficulties,resulting in the patients life quality decreased.Although the diagnosis technology and various treatment methods have made great progress in recent years,according to their clinicopathological characteristics.However,studies have found that the prognosis of tongue squamous cell carcinoma is poor after treatment.Studies have shown that human mesenchymal stem cells have been proved to play an anti-inflammatory,immunoregulatory and repair role in various animal models,and mesenchymal stem cells can migrate to many kinds of tumors.But at present,HUC-MSCs has a mixed effect on malignant tumors,and there is no research about the effect of HUC-MSCs on human tongue squamous cell carcinoma.Therefore,we will study the role of HUC-MSCs in the transplantation of human tongue squamous cell carcinoma.Methods:cal-27 cells were transplanted into BALB/c nude mice.Three weeks after we touch tumor,We injected HUC-MSCs with GFP green fluorescent protein labeled by tail vein.Then we study the effect of HUC-MSCs on the growth of the transplanted tumor.In order to clarify the mechanism,We analyze the relative m RNA expression on cell proliferation and apoptosis proteins bcl-2?bax?c-Myc??-catenin of the tumor tissue in the positive test group and the positive control group by q-PCR experimental.Results:In the model of human tongue squamous cell carcinoma in advance,HUC-MSCs labeled with GFP green fluorescent protein can be enriched in tumor tissues and inhibited tumor growth by intravenous injection of caudal vein.Through the q-PCR experiment,we found that compared with the positive control group,in the positive test group the m RNA expression of bax was up-regulated,and the expression of bcl-2??-catenin and c-Myc was down regulated in tumor.It was speculated that HUC-MSCs could inhibit tumor growth by promoting the formation of a homologous dimer of bax/bcl-2 and inhibiting the Wnt/beta-catenin signaling pathway.Conclusions:Through tongue squamous carcinoma nude mouse transplantation tumor model is established,The study found that GFP was expressed in a large number of tumor tissues.HUC-MSCs can inhibit the growth of tongue squamous cell carcinoma in vivo.