Effects Of Hehodge Signaling Pathway On Proliferation, Apoptosis And CyclinD1 Expression Of Breast Cancer Cell Line MDA-MB-231

Objective: The study was to research effets of HH singaling pathway on proliferation, apoptosis and CyclinD1expression of Breast cancer MDA-MB-231 cells. By MDA-MB-231 cells treated with Cyclopamine, that is a HH singaling pathway inhibitor.Methods: MDA-MB-231 cells were treated with Cyclopamine.Cell proliferation was detected by MTT;cell cycle distribution and apoptosis were analysed by flow cytometry (FCM).The mRNA levels of GLI1,CyclinD1 were measured by reverse transcription-polymerase chain reaction (RT-PCR);the protein level of CyclinD1 was detected by immunostaining of cell lines.Results: Compared with control group,Cyclopamine could significantly inhibit the proliferation of MDA-MB-231 cells in a dose- and time-dependent manner;by FCM the treatment of MDA-MB-231 cells with Cyclopamine induced a remarkable increase in the proportion of cells in the G0/G1 phase of the cell cycle and after 48h the sub- G1 peak appeared.The mRNA leves of GLI1,CyclinD1 and the expression of CyclinD1 protein were reduced.Conclusions: The HH signaling pathway was actived in MDA-MB-231 cells. HH signaling pathway inhibitor,Cyclopamine, could inhibit the proliferation,induce the apoptosis and reduce the expression of CyclinD1 in MDA-MB-231 cells.