Effect Of Radiotherapy On NK Cells And Related Immunocytokines In Peripheral Blood Of Patients With Lung Cancer

Aim:To investigate the changes of NK cell activity,IL-12,IL-15,IL-18 and s MICA levels in patients with lung cancer before and after radiation therapy.The relationship between radiotherapy efficacy and the changes of IL-12,IL-15,IL-18 and s MICA concentrations was analyzed.Method:Screening for lung cancer patients who first visited the Department of Oncology at the Affiliated Hospital of Chengde Medical College from April 2017 to January 2018 to include lung cancer patients who were identified as inoperable by histopathology and imaging,and before radiotherapy,radiotherapy 2 Week,radiotherapy for 4 weeks,radiotherapy for 6 weeks of peripheral blood.MACS was used to detect the activity of NK cells.Enzyme-linked immunosorbent assay was used to detect the levels of IL-12,IL-15,IL-18 and s MICA.Statistical analysis was performed using spss19.0 software.The data were normality test,IL-12,IL-15,IL-18,s MICA,NK cell activity before and after radiotherapy data analysis using single factor repeated measurement analysis of variance.The change of cytokine concentration before and after radiotherapy between different therapeutic effects was examined by two-factor repeated measurement analysis of variance.P <0.05 was considered statistically significant.Result:1.The concentrations of NK cells,IL-12,IL-15,IL-18 and s MICA before and after radiotherapy were increased along with the increase of the dose of IL-radiotherapy meter before and after radiotherapy(2 weeks,4 weeks,6 weeks).P <0.05).Before radiotherapy,the concentration of s MICA at 2 weeks,4 weeks and 6 weeks after radiotherapy decreased gradually with the increase of radiotherapy dose(P <0.05).Before radiotherapy,the concentration of IL-18 at 2 weeks,4 weeks,and 6 weeks after radiotherapy did not change significantly with the dose of radiotherapy(P> 0.05).The activity of NK cells was before radiotherapy and 2 weeks and 4 weeks after radiotherapy.At 6 weeks,there was no significant change in the activity with radiation dose(P> 0.05).2.Efficacy and radiotherapy before and after radiotherapy,2 weeks,4 weeks after radiotherapy,2 courses of treatment and radiotherapy before and after IL-12,IL-15,IL-18,s MICA concentration changes tumor shrinkage and tumor stability on IL-6 weeks concentration There was no significant difference in changes(P> 0.05).Conclusion:1.Radiation has no significant effect on the activity of NK cells in the peripheral blood of lung cancer patients,so radiotherapy can not affect the killing effect of NK cells on tumor cells.Radiotherapy can increase the levels of IL-12 and IL-15 in peripheral blood of patients with lung cancer,and further activate the patient's immune function.3.Radiotherapy can reduce the s MICA concentration of tumor antigen in patients' peripheral blood,indicating that the tumor escape function is inhibited and indirectly enhance the body's immune function.