Effect Of Chlamydial Protein Pgp3 On Skin Lesions、IL-17、IL-22 And IFN-γ In Psoriasis Mouse Model

[Objective] To observe the effect of chlamydia protein Pgp3 intervention on skin lesions improvement and the expression level of IL-17,IL-22 and IFN-γ in mouse models of psoriasis.[Methods] The fusion protein GST-Pgp3 was expressed by Escherichia coli,the protein was purified by glutathione S-transferase(GST)Magbeads,and the GST label was excised by PreScission protease to obtain the protein Pgp3.Twenty-four BALB/c female mice weighing 18-20 g and 6-20 weeks were randomly and equally divided into 6 groups:blank control group,model group,Ext-C group,Ext-H group,Ext-L group,CT795 protein group.In addition to the blank control group,the other 5 groups of mice were induced with imiquimod(IMQ)in a model of psoriatic lesions.In addition to the blank control group and the model group,the other 4 groups of mice were intervention: 200μl gelatin for external use in the control group,200μl protein pgp3 for external use in Ext-H group with the concentration is 1000 μg/ml,200μl protein pgp3 for external use in Ext-L group with the concentration is 50 μg/ml,and 200μl protein CT795 for external use in Ext-C group with the concentration is 1000 μg/ml..During the experiment,the changes in the lesion area of the mouse’s back were observed daily,and changes in the skin lesions of the mice were recorded daily according to the psoriasis model(TSS)score.At the end of the experiment,the mice were executed and the lesions were taken from the mice.Some of them were sectioned by pathology,and the morphological changes of the skin lesions in each group were observed under a microscope.Some of the tissues were treated with Real Time PCR(Real-time Polymerase).Detection of IL-17,IL-22,and IFN-γ RNA expression level in mouse skin lesions and analysis of changes.[Results] The lesions of mice treated with topical Pgp3 protein group were significantly alleviated compared with the model group,control group,and topical CT795 protein group.The TSS scores were also lower than the other groups.The results of Real-Time PCR showed that the expression of IL-17,IL-22 and IFN-γ in skin lesions of mice treated with topical Pgp3 protein group was lower than that of model group and CT795 protein group,and the difference was statistically significant(P<0.01).[Conclusion] In the mouse model of psoriasis,chlamydia protein Pgp3 can inhibit the development of psoriatic lesions in mice,and can down-regulate the expression of IL-17,IL-22 and IFN-γ.