Study On Antibacterial Properties And Cell Compatibility Of Antibacterial Peptide Coating Of Titanium Implants

Objective :To endow the surface of Ti dental implant with antibacterial property and biological activity,the influences of antimicrobial peptide Pac-525 coatings on porphyromonas gingivalis’ s antibacterial performance and the osteoblast proliferation and adhesion were discussed.It provides a basis for the assembly of antibacterial peptides on the surface of titanium implant materials and loading,connecting and constructing functional biological coatings with antibacterial function and good bioactivity.Methods :After ultrasonic micro arc oxidation,alkali treatment and silane treatment of pure titanium,0.25,0.50,0.75 g/L antibacterial peptide Pac-525 were added to the antibacterial peptide group(each group has 45),the titanium specimens in the control group were only treated by ultrasonic micro arc oxidation,alkali treatment and silaning treatment.The morphologies of coatings were observed by scanning electron microscope,and the determination of element changes were detected by energy spectrum analyzer and the ratio of the living bacteria,orange acridine-ethidium bromide double staining was used to detect the average percentage of live bacteria and biofilm thickness,after the specimens of each group and Porphyromonas gingivalis were cocultured with 72 h.Cell Counting Kit-8 method and immunofluorescence staining were used to test the proliferation of osteoblasts,the number and growth morphologies of adherent cells,respectively.Results :Scanning electron microscopy and energy spectrum analyzer showed that the pac525 particles loaded on the surface of the coating,and the C and N elements in the Pac-525 coating group were significantly more than those in the control group.The average percentage of living bacteria in the control group,0.25,0.50 and 0.75 g/L antimicrobial peptides were 0.58%,0.45%,0.34% and 0.28%,respectively,the difference between each group was statistically significant(P<0.05).The biofilm thickness of Porphyromonas gingivalis in 0.50 and 0.75 g/L antibacterial peptide group was(98.30±1.20)and(94.54±2.49)μm] respectively,which were significantly less than those in control group and 0.25 g/L antibacterial peptide group [(117.63±1.46)and(117.99±1.29)μm](P<0.05),respectively;the number of bone cell adhesion and proliferation of all antimicrobial peptides were significantly greater than those of the control group(P<0.05),and the cells were well spread out.Conclusions :The antibacterial peptide coating of titanium implants could inhibit the formation of bacterial biofilm.It had good antibacterial properties and could promote the adhesion and proliferation of osteoblasts.