Association Of Polymorphisms In Long Non-coding RNA HOTAIR And PRNCR1 With The Risk Of Gastric Cancer

Objective:The formation of gastric cancer?GC?is a multigene process.Long non coding RNAs?LncRNAs?HOTAIR and PRNCR1 are closely related to the development of cancer.SNP refers to the sequence polymorphism of single nucleotide in DNA sequence.As the most common genetic variant,SNP is common in lncRNAs.In this study,the relationship between LncRNA and gastric cancer susceptibility was discussed by studying the SNP of HOTAIR and PRCNR1,and new ideas for future risk prediction and early diagnosis of gastric cancer was provided.Method:Blood samples of 92 patients with gastric cancer were collected and 56cases of healthy control blood samples were collected.Meanwhile,the gender,age,tumor site and degree of differentiation were collected.Extraction of genomic DNA:the extraction of blood samples from the gastric cancer case group and the control group.In the literature review,two SNP sites with clear correlation with the tumor were identified as the research object,and the relationship between them and gastric cancer will be discussed.The two sites were located in the long chain non-coding RNA HOTAIR and PRNCR1respectively.According to the sequence design of SNP,PCR amplifies the target segment of SNP.The target gene genotype was detected by Ligase detection reaction?LDR?technology.Statistical analysis:SPSS 21.0 statistical software was used for data analysis.P<0.05 was considered statistically significant.Results:1.There was no significant difference in gender and age between the gastric cancer group and the control group.2.In this study,41 patients were randomly selected in the gastric cancer group,and 56 cases in the control group were combined.HOTAIR rs2366152and PRNCR1 rs7463708 were amplified and sequenced in the two groups of samples.Results the frequency of alleles in rs2366152 could not be considered as statistically significant?P=0.507?.Rs7463708 had different allele frequencies?P=0.046?.The result were statistically significant,so the sample size of rs7463708 was expanded.3.A total of 92 cases of gastric cancer and 56 cases of control group were included in PRNCR1 rs7463708.There was a statistical difference between the two groups?P=0.009?.It was found that individuals with genotype GT had a71.3%lower risk of gastric cancer than those with genotype GG(P_adjusted=0.024).The risk of gastric cancer was reduced by 76.1%for individuals with genotype TT(P_adjusted=0.011).Individuals with genotype GT/TT genotypes had a 73.6%lower risk of gastric cancer than those carrying genotype GG(P_adjusted=0.011).At the same time,the risk of gastric cancer was reduced by 47.3%for the allele T?P=0.009?.Hardy-Weinberg balance test of control group P>0.05?P=0.502?.After stratified analysis,the results showed that rs7463708 can lower the risk of gastric cancer was correlated with age,different tumor location,differentiation degree and TNM staging.Individuals with GT/TT genotypes were compared with those with GG:the younger individuals??60 years old?had a 85.7%lower risk of gastric cancer?P=0.015?,the probability of upper gastric cancer was 74.4%lower(P_adjusted=0.041).The risk of distal gastric cancer decreased by 73.9%(P_adjusted=0.014).The probability of poorly differentiated gastric cancer decreased by 78.5%(P_adjusted=0.005).The probability of developing gastric cancer decreased by 74.4%(P_adjusted=0.013).Conclusions:1.For PRNCR1 rs7463708,the frequency of genotypes in the gastr ic cancer group was statistically different from that in the control group.allele T is a dominant allele to inhibit carcinogenesis of gastric cancer.2.It is not yet believed that HOTAIR rs2366152 single nucleotide polymorphism is associated with the risk of gastric cancer.