Phase Characteristics Analysis Of Blood Cell And Three-dimensional Reconstruction Method For The Typical Model

Blood cells are the main component of the blood system,which maintains the healthy operation of the blood system and is also closely related to metabolic activities such as nutrient intake of living bodies and waste excretion.Scientific research on blood cells has very important significance in the field of life sciences,especially in terms of species identification and feature analysis.The phase microscopic imaging technology has been used as an important means for blood cell microscopic imaging and structural detection because of its advantages of non-invasive.The imaging accuracy,real-time monitoring of blood cells,and quantification of experimental data are unmatched by traditional microscopic imaging technology.Phase imaging is generally based on optical holography theory,which mainly includes two major steps: phase image acquisition and phase recovery.The merits of phase recovery will directly affect the reconstruction of the phase.At present,most of the phase recovery require an unwrapping process,and this process will affect the efficiency and imaging accuracy,and the phase obtained by phase recovery can not truly reflect its 3D topography and subsurface structure distribution.In view of the above problems,based on reviewing and analyzing a large number of literatures such as phase feature analysis and 3D reconstruction algorithms,this paper focuses on the unwrapped phase recovery algorithm and the 3D reconstruction method under the typical model:1.In the aspect of unwrapped phase recovery.In order to solve the problem of large error caused by the unwrapping program and the limitation of application,a phase gradient method based on co-axis two-step phase-shifting interferometry is proposed in combination with phase shift theory and coaxial interference technique.The phase gradient can be obtained by only two interferograms.The morphological structure of the sample can be obtained without phase unwrapping.The simulations of the polystyrene spheres and red blood cells prove the effectiveness of the method.In addition,combined with dual-wavelength technology and carrier-frequency interferometry,a dual-wavelength unpacked phase recovery method under a single carrier frequency is proposed.This method only needs to complete the phase recovery through the spectrum filtering operation of a single interferogram.The simulation of monocytes verifies the feasibility of the theory.2.In the aspect of 3D reconstruction algorithm,because the traditional quantitative phase can not truly reflect the internal structure of the sample,the four peak Gauss function and the single angle phase diagram are taken as the object.The reconstruction effect of the three back projection reconstruction algorithms and the reconstruction precision of the two filter functions under the noise interference are explored and analyzed.The research work in this paper provides a reference for quantitative analysis of blood cells and three-dimensional imaging research has a certain application value.