MIF Expression Profile In Serum And Gingival Tissues From Patients With Chronic Periodontitis

Objective: Periodontitis is a bacterial infection characterized by the persistent destruction of periodontal tissue.Porphyromonas gingivalis(P.gingivalis)is the periodontal pathogens,not directly damage the periodontal tissue,but also drive the immune response and then stimulate the release of proinflammatory cytokines such as interleukin-1(IL-1),tumor necrosis factor alpha(TNF-?)and monocyte chemoattractant protein-1(MCP-1),further destruct periodontal tissue.Macrophage migration inhibitory factor(MIF)is a multifunctional cytokine,involved in many diseases,was found in saliva,gingival crevicular fluid and gingival tissues of patients with periodontitis,suggesting that MIF might have close relationship with periodontitis.This experiment aims to investigate the levels of serum MIF and MCP-1 in patients with chronic periodontitis and MIF and MCP-1 protein levels in gingival tissue,so as to provide new evidence for MIF in chronic periodontitis,further investigate the pathogenesis of chronic periodontitis,and provide a reference for treatment.Methods: 1.18 cases with severe chronic periodontitis and 18 persons with healthy periodontal conditions are selected from Stomatology Hospital of China Medical University,the serum was collected and stored at-70? until use.MIF and MCP-1 were measured by enzyme-linked immunosorbent assay(ELISA).2.Gingival tissue samples were collected from 8 cases with chronic periodontitis,18 cases with clinically healthy gingival tissue and 11 cases with chronic periodontitis who have received professional scaling and root planning for MIF and MCP-1 immunohistochemistry analyses.The expression of MIF and MCP-1 in gingival epithelia or underlying connective tissues was then calculated by using the immunohistochemical image analysis software.P.gingivalis was detected by fluorescence in situ hybridization within gingival tissues from healthy controls and patients with chronic periodontitis.The study protocol was reviewed and approved by the local ethics committee ethical review(2015-12).Participants were enrolled into the study after signing an informed consent.3.SPSS 24.0 statistical software package was used for all the obtained data.The clinical index were reported as mean±standard deviation.We used t-tests and ANOVA to compare the differences between groups.Results: 1.Serum MIF and MCP-1 were signifcantly higher in chronic periodontitis patients compared with healthy controls,the difference was statistically significant(P<0.05).2.MIF protein was expressed in gingival epithelia,and the MIF level was highest in periodontitis gingiva,and its level was decreased by scaling and root planning,while the expression of MIF in healthy gingiva was the lowest(P<0.001);MCP-1 protein was also expressed in both gingival epithelia and connective tissues,but there was no significant difference between the periodontitis and healthy control(P >0.05).Conclusion: As an inflammatory factor,MIF may be involved in the progression of chronic periodontitis,and MIF level may be used as an auxiliary marker for the diagnosis and curative effect of chronic periodontitis.