The Research About The Role Of Hax-1 In Cell Apoptosis Induced By Endoplasmic Reticulum Stress

ObjectivesTo investigate the effect of Hax-1 on apoptosis of cells induced by Endoplasmic Reticulum stress(ER stress).Methods1.The Endoplasmic Reticulum stress model in cells was induced by Tunicamycin in vitro,and the apoptosis of cells and expression of Hax-1 were detected.2.A recombinant plasmid of human Hax-1 was constructed,and its high expression in cells was confirmed.3.Cells were treated with Tunicamycin after the transfection of Hax-1,and the role of Hax-1 in apoptosis induced by ER stress was explored.Results1.In the apoptosis induced by ER stress,protein level of Cleaved-Caspase-3 increased significantly,while Hax-1 decreased dramatically.2.Overexpression of Hax-1 could lessen the production of apoptin,partly restrain apoptosis of cells induced by ER stress,and stabilize mitochondrial membrane potential(MMP).ConclusionOverexpression of Hax-1 partly restrain ER stress-induced apoptosis.