The Observation Of The Acute Inflammatory Reaction After Titanium Samples With Different Surface Morphology Implantation

When implants are implanted in the body,the tissue injury caused by the operation and the foreign body stimulation of the implant will induce the foreign body reaction?FBR?^?1.2?.The severity of FBR and its outcome effect on the life and function of implant.Acute inflammatory reaction phase is the early stage of FBR,and its severity and outcome will affect the subsequent stage of FBR.In order to improve the titanium osseointegration,our team have fabricated the hierarchical micro/nano-textured topography on the titanium surface andproved that this morphology can improve the osseointegration of metal titanium in vivo and vitro study^?3?.Although our team have made a deep exploration of the mechanism of theimprovement capacity,we are knowing nothing about the influence of micro/nano-textured topography on acute inflammatory response after implanted^?5.6?.In order to clear whether the micro/nanotopography surface influence the acute phase of inflammation after implanted and have a more particular knowledge about the mechanism,we design the following research.In this research,we fabricated different surface morphology on titanium samples by the means of sanding,etching andanodization.Then,we tested the osseointegration of the titanium samples with different surface morphology after implanted in C57BL/6mouse by Micro CT,hard-tissue slicing and Van-Gieson staining and press-in strength test.Last,after implanted in subcutaneous,enterocoelia and thighbone ofC57BL/6 mouse we observed the acute inflammatory reaction by the means of microtomy,flow cytometry and protein-chip technique.Result:1.The smooth surface of sandpaper were visible only some sligthly scratch under the electron microscope.The micro pit morphology,fabricated by HF solution etching,were full of micron-sized pit.The micro/nano-textured topography,fabricated by anodization on the foundation of micro pit morphology,were full a lot of uniform nanotubes under the scanning electron microscopy.2.The osseointegration around titanium samples with micro/nano morphology were significantly better than those of samples with micro pit morphology and smooth surface in the mouse.The osseointegration around titanium samples with smooth surface were worse than others.3.After implanted 16 and 72 hour,the acute inflammatory,reaction induced by titanium samples with micro/nano morphology,were slightest.And meanwhile,the acute inflammatory,reaction induced by titanium samples with smooth surface,were the most serious.In general,the acute inflammatory reaction after titanium samples implanted 72hour Notablely lighten in comparison with 16 hour.Conclusion:3.Titanium with micro/nano morphology could promote the formation of osseointegration,and the micro-nano morphology with different nanotube diameter could influence their improvement function.4.Titanium with micro/nano surface morphology can inhibit the acute phase inflammatory reaction after implantation,and the inhibitory effect of different nanotube diameter on the acute phase inflammatory reaction were different.Thus,the hypothesis could be obtained.The micro/nano morphology of the titanium surface could influence the formation of the final osseointegration by affecting the acute inflammatory reaction.This study has enriched the evidence of the influence of implantation material morphology on the acute inflammatory reaction stage of FBR,and has made some exploration on the mechanism of titanium micro/nano morphology promoting osseointegration.