The Study Of B Cell Subsets And Immune Globulin In Patients With Blood Stasis Syndrome Of Coronary Heart Disease

Background:Coronary heart disease(CHD)is mainly caused by atherosclerotic.Atherosclerotic lesions cause vascular stenosis or obstruction,causing myocardial ischemia,hypoxia,or necrosis.CHD seriously affects human physical and mental health and is one of the major factors leading to death.Different cells of the immune system,including lymphocytes and macrophages,are involved in the formation and development of atherosclerosis.Different B lymphocyte subsets have different roles in atherosclerosis.CHD belongs to the category of "brachial fistula","heartache" in Chinese medicine.Blood stasis syndrome is the main syndrome of CHD.It is of great significance to study the role of blood stasis syndrome in the occurrence and development of CHD.Objective:To investigate the level of B-lymphocyte subsets in peripheral blood of patients with CHD and healthy people,fiow cytometry(FCM)was used to detect B-lymphocyte subsets.We observed and compared the differences between CHD patients and health control and differences between CHD with and without blood stasis syndrome.On the other hand,we observed the levels of serum IgA,IgG,and IgM in the peripheral blood of the investigators.And then we intended to explore the correlation between different B cell subsets,IgA,IgQ and IgM and CHD,CHDwith.blood stasis syndrome and provide reference indexes for the syndrome differentiation of CHD.Methods:We selected 98 CHD patients from September 2017 to February 2018 in Guang'anmen Hospital,a total of 30 healthy volunteers were selected as the control group.The patient with CHD were divided into blood stasis syndrome group non-blood stasis syndrome group.In an early morning fasting state venous blood was collected from cubital vein,and collect peripheral blood serum and heparin anticoagulant plasma samples.Heparin anticoagulant plasma samples were used to measure peripheral blood B lymphocyte subsets by flow cytometry;peripheral blood serum samples were used to test the level of IgA?IgM?IgG.The collected data was processed and analyzed by the statistical analysis software.Results:98 CHD patients and 30 healthy subjects were included in the study.49 CHD patients with blood stasis syndrome and 49 CHD patients with non-blood stasis syndrome.After statistical analysis,there was no significant difference in age,sex ratio,blood sugar,liver and kidney function between the two groups of CHD patients and the physical examination group.There was no significant difference in age,sex ratio,blood sugar and liver and kidney function between CHD patients with blood stasis syndrome and non-blood stasis syndrome.The level of CD 19+ cells in CHD patients was significantly higher than that in the healthy group,and the difference was statistically significant(P<0.01).The level of B10 and B1a cells in CHD patients was significantly lower than that in healthy group,the difference was statistically significant(P<0.05);the level of B1b cells in CHD patients was not significantly different from that in healthy group(P>0.05).The level of FOB cells in CHD patients was significantly higher than that in healthy group,the difference was statistically significant(P<0.05).There was no significant difference in MZB cell level between CHD patients and healthy group(P>0.05).The expression level of serum IgA,IgG and IgM in CHD patients were significantly higher than that in the healthy control group,the difference was statistically significant(P<0.05).The level of CD 19 positive cells in CHD patients with blood stasis syndrome was significantly higher than that of the non-blood stasis syndrome group(P<0.05).The level of B10 and B1a cells in CHD patients with blood stasis syndrome was significantly lower than that in CHD patients with non-blood stasis syndrome,the difference was statistically significant(P<0.05);there was no significant difference in B1b cell level between CHD patients with blood stasis syndrome and non-blood stasis syndrome(P>0.05).The level of FOB cells in CHD patients with blood stasis syndrome was significantly higher than that in CHD patients with non-blood stasis syndrome(P<0.05).There was no significant difference in MZB cell level between CHD patients with blood stasis syndrome and non-blood stasis syndrome(P>0.05).The expression level of serum IgA,IgG and IgM in CHD patients with blood stasis syndrome were significantly higher than in non-blood stasis syndrome group(P<0.05).Conclusion:1.The level of B lymphocyte and FOB cells in peripheral blood was increased in CHD patients.The levels of B10 and B1a cells in peripheral blood of CHD patients were decreased.2.The serum levels of IgA,IgG and IgM in the peripheral blood of CHD patients were increased.3.The level of B lymphocyte and FOB cells in peripheral blood of CHD patients with blood stasis syndrome was increased.The level of B10 and B1a cells in peripheral blood of CHD patients with blood stasis syndrome was decreased.4.The levels of IgA,IgG and IgM in peripheral blood serum of CHD patients with blood stasis syndrome were increased.