Study On Platelet Microparticle Function And Cell Microarray Binding In Patients With Coronary Heart Disease And Angina Pectoris And Blood Stasis Syndrome

Objective:The CD62p, PAC-1, CD42b and CD40L expression levels on peripheral blood platelet microparticles (PMPs) membrane surface was detected by flow cytometry to investigate the differences in the patients with different severity of coronary heart disease unstable angina pectoris(UA). On the other hand, we obsevered the aggregation formed between PMP and leukocyte microparticles (IMP), endothelial microparticles (EMP), red cell particles (RMP) and detected the aggregation levels in the patients with different severity of UA by the imaging flow cytometry. At the same time, we investigated the levels of PMP surface membrane functional protein, PMP-LMP aggregation, PMP-EMP aggregation, PMP-RMP aggregation between coronary heart disease UA blood stasis and non blood stasis syndrome, providing a theoretical basis for the formation of blood stasis syndrome.Methods:We selected 110 CHD patients from August 2015 to December 2015 in Guang’anmen Hospital, used 20 healthy volunteers from Southern District of Guang’anmen Hospital as a control group. The patients with coronary heart disease were divided into blood stasis syndrome group and non blood stasis syndrome group, and they were divided into I grade, II grade and III grade according to Braunwald classification. All subjects’blood were save by sodium citrate anticoagulation. Using gradient centrifugation method for preparing platelet-free plasma, the PFP were kept in-80℃ refrigerator and tested in two weeks by flow cytometry. The levels of PMP and its membrane surface CD62p, PAC-1, CD42b, CD40L expression was detected by flow cytometry, and PMP-LMP aggregation, PMP-EMP aggregation, PMP-RMP aggregation was detected and their morphology was observed by imaging flow cytometry. We analysed of correlation relationship between these indexes and the severity of coronary heart disease unstable angina and coronary heart disease with blood stasis syndrome.Result:1. The expression level of PMP and CD62p and PAC-1, CD40L in patients with UA is higher than healthy people, the difference is statistically significant (P< 0.05);2. The expression level of PMP and CD62p and PAC-1, CD40L in patients with blood stasis syndrome is higher than non blood stasis syndrome, the difference is statistically significant (P< 0.05); and they were positively correlated with blood stasis syndrome;3. The levels of PMP in the patients in UA level III group is higher than patients in UA level I, level II group, the difference is statistically significant. PAC-1, CD40L expression levels in the patients in UA level II and level III group were higher than those in patients in UA level I group (P< 0.05), CD62P expression level in patients in UA level III group is higher than the patients in UA level I and II group (P< 0.05). Logistic regression analysis showed that the expression level of PMP and CD62p, PAC-1, CD40L were closely related to the severity of unstable angina;4. PMP, LMP, EMP, RMP, PMP-LMP aggregation, PMP-EMP aggregation, PMP-RMP aggregation in patients with UA was higher than the level of healthy people, the difference was statistically significant (P< 0.01);5. PMP, RMP level in patients in UA III level group was higher than those of in patients in UA level I and II group, the level of LMP, EMP, and PMP-EMP aggregation in patients in UA II and UA III group was higher than patients in UA level I group, the difference was statistically significant (P< 0.05);6. The expression level of PMP, LMP, EMP, RMP, PMP-EMP aggregation, PMP-RMP aggregation in patients with blood stasis syndrome is higher than non blood stasis syndrome, the difference is statistically significant (P< 0.05); and they were positively correlated with blood stasis syndrome(P< 0.01).Conclusion:1. The level of PMP, CD62p, PAC-1 and CD40L in UA patients was higher than healthy people, and these indexes were related with the severity of UA, suggesting that the function of adhesion, aggregation, promoting inflammation of PMP involve in the occurrence and development of UA.2. The level of PMP, CD62p, PAC-1 and CD40L in UA patients with blood stasis syndrome was higher than non-blood stasis syndrome patients, suggesting that the function of adhesion, aggregation, promoting inflammation of PMP involve in the formation of blood stasis syndrome.3. PMP-LMP aggregation, PMP-EMP aggregation, PMP-RMP aggregation in patients with UA was higher than the level of healthy people, and they were positively correlated with the severity of UA.4. The level of PMP, LMP, EMP, RMP, PMP-EMP aggregation and PMP-RMP aggregation in UA patients with blood stasis syndrome was higher than non-blood stasis syndrome patients, and these indexes were positively correlated to blood stasis syndrome, suggesting PMP, LMP, EMP, RMP, PMP-EMP aggregation and PMP-RMP aggregation play an important role in the formation of blood stasis syndrome.