| Objective:In this study,we observed the effect of different processing technology of Radix Rehmannia saponin extract on the ovulation function of female rats,observed the estrus cycle using vaginal cell smear,and took the bilateral ovary for pathological examination.Estradiol was detected by ELISA technique.),The content of progesterone(P)hormones,compared the differences in the efficacy of rehmannia yin and kidney stimulating ovulation with different processing techniques.At the same time,based on reversed-phase high performance liquid chromatography,the main chemical constituents of Rhizoma rehmanniae prepared by two kinds of processing technology were determined(raucoside,5-hydroxymethylfurfural,mothertoside)content.To establish the characteristic map of the process of nine steaming and curing of Rehmannia,and to use the bivariate correlation analysis method to combine the effect of the two processing technologies of Rehmannia glutinosa and ovulation.This study combines data from chemical and pharmacological studies and compares the superiority of nine-steaming and one-time steaming processing techniques for rehabilitated land.It provides experimental data for the scientific connotation of Rehmannia sorghum,ancient method nine-steaming,and nine-steam processing,and guarantees reliance on clinical drug use.The processing quality of the decoction pieces has certain guiding significance.Methods:1 Study on Processing Techniques of Radix Rehmanniae Prepared by Ancient and Modern Techniques and Preparation of Medicinal HerbsThrough the library's search of ancient Chinese books related to ancient concoctions,the regulations for the preparation of Chinese herbal medicines in various provinces and municipalities,the teaching materials for"Chinese medicine processing"and field investigations,some pharmaceutical companies in the field have obtained relevant information about cooked Radix Rehmanniae Preparata,combined with the conditions of the laboratory's own facilities,Under the guidance of Associate Professor Xia Xi of the School's Teaching and Research Section,this experiment was conducted on the preparation of the cooked yellow rejuvenation method and the traditional method.2 Pharmacodynamic study of the effect of two processed techniques of Radix Rehmanniae on ovulation in ratsNormal female adult rats were randomly divided into blank group,steamed group and traditional group.The mice in the drug-administered group were given 3.6g·kg~-11 gavage of decoction of decoction yellow,and the blank group was given the same amount of saline.Observed the effects of the two decoctions of Rehmannia radix decoction on the estrous cycle,ovarian morphology and serum hormone content in female rats,and explored the influence of different decoctions of Radix Rehmanniae decoction on ovulation of female rats.3 Determination of the main chemical constituents of two kinds of Rehmannia glutinosaThe water extracting and alcohol precipitation method was used to remove the interference of polysaccharides and sugars to prepare the rehmannia root aqueous extract.Based on reversed-phase high performance liquid chromatograph technology,a HPLC method was established for the determination of verbascoside,5-hydroxymethylfurfural,and mothertoid,two main chemical constituents of Radix Rehmanniae prepared by steaming method and traditional method.Three chemical components in water extracts were determined.The content changes.4 Fingerprinting and Spectroscopy Study of Cured Land ProcessingThe water extracting and alcohol precipitation method was used to remove the interference of polysaccharides and sugars to prepare the rehmannia root aqueous extract.Based on reversed-phase high-performance liquid chromatography,fingerprints of two rehmannia glutinosa extracts and nine evaporation processes were studied in order to observe the changes of the chemical components of Radix Rehmannia glutinosa during processing.According to the chromatographic relationship between chromatographic peak areas and pharmacodynamic indexes of each feature of the fingerprint spectrum,the effective substances related to ovulation in the two processing technologies of Rehmannia glutinosa were explored.Result:1.Rehmannia ancient processing technology and medicine processingCheck the ancient books 6,the provinces and autonomous regions in a total of seven Chinese herbal concoction specifications,traditional Chinese medicine teaching a total of three volumes of books,teaching under the guidance of teachers,the experiment developed steamed Rehmannia glutinosa process for a one-time steamed12 h removed,Drying oven at 60?,drying time of 4 h,cut 2-4 mm thick slices,sealed.The traditional method of processing:steam 6h,removed and placed in a cool place,the sun in a sunny place the next day to 7 hours,before the second steaming,mix in the last due to steam generated Rehmannia juice,steamed again 6 h,if Repeated 8 times,a total of 9 times,cut 2-4 mm thick slices,sealed and saved.Traditional law Rehmannia black shiny,oily shiny,sweet.Rehmannia Rehmannia,sweet sour,gloss is not obvious,the black is still light,obvious bitterness.2.Pharmacodynamics of two kinds of processing technology Rehmannia glutinosa on ovulation function in ratsEighteen female rats were randomLy divided into 3 groups,which were recorded as traditional group,steamed group and blank group,6 in each group.Vaginal smear recorded a total of four sexual cycles,the results showed that the traditional group,the blank group cycle was not obvious,the average number of days was 4.3 days,and steamer group cycle gradually increased after the second sexual cycle,the average number of days were 4.50,5.83,7.00,7.17 days,significantly greater than the traditional group and the blank group(P<0.05).BX41 microscope photograph(×40)The follicles in the traditional group and the blank group were normal in morphology,and the corpus luteum and the secondary follicle were clearly visible.Steamed follicles more follicles atresia follicles,and luteal and secondary follicles blurred.The numbers of atresia follicles in the steamed group,the traditional group and the blank group were(4.0±0.6),(2.2±0.5)and(2.3±0.5),respectively.Follicles in the steamed group were significantly more than the conventional group and the blank group(P<0.05).(17.21±0.19)and(5.39±0.11)in the blank control group(17.36±0.24)and(5.41±0.19),respectively,and that of the steamed group were(10.69±0.22),(3.08±0.30).There was no difference between the blank group and the traditional group.The content of the blank group was significantly lower than that of the blank group and the traditional group(P<0.05).3.The main chemical composition of Radix Rehmanniae DeterminationTwo kinds of processing technology were obtained by water extraction and alcohol ablation of polysaccharides.Two steps of steaming rehmannia glutinosa rehmanniae and nine steaming nine dry Rehmannia glutinosa water extracts were obtained.The mass fractions of verbascoside in the samples were 0.097%,0.033%and 5-HMF respectively Respectively,0.15%and 0.66%respectively.The mass fraction of motherwort glycosides in the samples were 0.587%and 0.209%,respectively.4.Rehmannia glutinosa process fingerprinting and spectroscopy studyRehmannia glutinosa process during a steamed to nine steam nine sun nine samples,modern steamed law Rehmannia and Rehmannia samples a total of 11batches of samples to do fingerprinting.A total of 9 peaks were observed,of which,the overall trend of peak areas of 2,3,4,and 5 increased with the increase of processing times.6,7,8,9 peak in the initial processing peak area value,peak area change on the 1st is not obvious.Peak area of the steamed group was compared with that of the traditional group,and the cycle data of the vaginal smears recorded in the pharmacodynamics and the data of the atretic follicles were combined.Using the bivariate correlation analysis method to do the pedigree study,the results showed that No.1,8 peak and ovulation suppression Positive correlation3,4,5 peak negative correlation with inhibition of ovulation.Conclusion:Traditional Law(nine steam nine sun)Rehmannia there is evidence that the market circulation to rehmannia Rehmannia mostly.In the appearance of medicinal herbs,taste evaluation on nine steam nine dry Rehmannia more advantages.Stewing lawn cooked Rehmannia on normal female rats ovulation have a certain inhibitory effect,traditional law Rehmannia oviposition function has some advantages.After processing by different processes,the main chemical constituents of Rehmannia glutinosa,5-HMF and other significant changes occur,the spectroscopic studies have shown that the relevant chemical composition and inhibition of ovulation there is a certain relationship. |
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