Study On The Mechanism Of Treating Rabbits Knee Osteoarthritis By Dissolving MAPK Signaling Pathway Through Meridian-sinew Release Therapy

PurposeThrough the experimental study on the treatment of rabbit knee osteoarthritis with Meridian-Sinew Release Therapy,to explore the effect of this treatment on MAPK signal transduction pathway and further explore its mechanism.MethodGrouping: 24 New Zealand rabbits were randomly divided into control group(n = 6)and experimental group(n=18).The experimental group was randomly divided into Meridian-Sinew group,acusector group and model group after 45 days of modeling.Modeling: 24 New Zealand rabbits received needles from the depression of the outer knee at the first,fourth and seventh days of the experiment.the experimental group was injected with 0.5 ml of papain 3%,and the control group was injected with the same volume of normal saline for 3 times.Treatment: control group: normal feeding,no treatment;model group: normal feeding,no treatment;acusector group: select Yanglingquan,YinLing-quan,inside and outside knee eye acupoints for acusector treatment,every other day,the total course of treatment is 1 month;In the Meridian-Sinew group,the left knee joint was treated with endoscope for 1 week first,then treated with needle knife once a week,3 times a week,for a total course of one month.Rabbits in each group were sacrificed by intravenous air embolism one week after the end of treatment.The left knee joint was dissected to observe the lesions of cartilage and capsule of the left knee in each group.The expression of MMP13,ERK1/2 and P38 genes in articular cartilage was detected by realtime fluorescence quantitative PCR,and the expression of MMP13,p-P38,p-ERK1/2,and Collagen II proteins was detected by Western Blot.SPSS 22 software was used for statistical analysis of data.ResultObservation: the control group: there was no red swelling in left knee joint;no formation of vasospasm,smooth and translucent articular surface;no enlargement and hypertrophy of the joint capsule;model group: The left knee joint was obviously red and swollen;The articular cartilage is dim and dull,and the partial bone surface is rough,the defect is obvious,and the joint capsule is thickened;the Meridian-Sinew group : left knee joint slightly swollen;The cartilage is pale and white,the bone surface is slightly worn,and the joint capsule is slightly thickened;Acusector group : left knee joint slightly swollen;The cartilage is pale and white,the bone surface is slightly worn but more severe than the muscle group;the joint capsule was mild thickened.The expression of MMP-13,P38,and ERK1/2 genes in articular cartilage was detected by fluorescence quantitative PCR: There was no significant difference between the four groups(P>0.05).The MMP-13 gene was expressed as model group> acusector group> Meridian-Sinew group> control group,the MMP-13 gene was less expressed in Meridian-Sinew group,compared with acusector groups and the model group,the difference is statistically significant(P<0.05).The results of Western Blot test showed that the protein expression of p-P38,p-ERK1 and 2MMP13 in the three groups was higher than that in the control group,and the expression of p-P38,p-ERK1 and 2MMP13 in the Meridian-Sinew group was lower than that in the acusector group and the model group,the difference is statistically significant(P<0.05).ConclusionThe effect of Meridian-Sinew Release Therapy on knee osteoarthritis in rabbits was better than that of acusector.The possible mechanism was that by inhibiting phosphorylation of MAPK pathway and decreasing the expression of MMP-13 gene and the expression of type ? collagen protein.Therefore,the destruction of KOA articular cartilage can be effectively alleviated,and the treatment of knee osteoarthritis is effective.