Effect Of High Expression Of Vimentin On Migration And Invasion Of Nasopharyngeal Carcinoma CNE2 Cells

[Objective] The previous study of the research group found that LMP1 can induce EMT in CNE2 cells of nasopharyngeal carcinoma.LMP1 up-regulates the expression of vimentin.This study aims to investigate the effect of high expression of vimentin on migration and invasion of nasopharyngeal carcinoma CNE2 cells and provide experimental data for elucidating the molecular mechanism of nasopharyngeal carcinoma.[Methods] 1 Construction of pc DNA3.1(+)/Vimentin vector and transient transfection of nasopharyngeal carcinoma CNE2 cells with pc DNA3.1(+)as control,and detection of Vimentin Expression in CNE2 Cells of Nasopharyngeal Carcinoma by Western Blot;2.The effect of high expression of Vimentin on the morphology of nasopharyngeal carcinoma CNE2 cells was observed by light microscope,Western blot was used to detect the expression level of these two EMT-related proteins TGF-β and E-cadherin in nasopharyngeal carcinoma CNE2 cells after Vimentin overexpression..;3 The effects of high Vimentin expression on migration and invasion of nasopharyngeal carcinoma CNE2 cells were examined by wound healing assay and Transwell migration and invasion assay.[Results] 1.Expression of Vimentin in Nasopharyngeal Carcinoma CNE2 Cells Increased Significantly after Transient Transfection with pc DNA3.1(+)/Vimentin Vector,CNE2 cells are transformed from an Cobblestone-like epithelial cell morphology to a Spindle mesenchymal cell morphology,undergoing EMT-like cytomorphological changes.2.The results of Western blot showed that the expression of Vimentin in CNE2 cells was increased after transient transfection with pc DNA3.1(+)/Vimentin Vector,and the expression of TGF-β was also significantly increased,while the expression of E-cad was significantly decreased(P<0.05).3.wound healing assay results showed that the migration distance of CNE2 cells in the Vimentin high expression group was significantly greater than that in the empty vector control group and the untransfected group(P<0.05),The results of Transwell migration and invasion assay showed that the mobility and invasion rate of CNE2 cells in the high expression group of Vimentin were higher than that of the empty vector control group and the untransfected group(P<0.05).[Conclusions] Vimentin up-regulates the expression of TGF-β,downregulates the expression of E-cadherin,induces EMT in CNE2 cells,and enhances the migration and invasion ability of CNE2 cells.