Research On Inorganic Porous Composite Nanomaterials And Relevant Biosensing Properties

In recent years,due to the ordered porosity,large specific surface area,good adsorbability and the optimization and promotion of preparation methods,nano-porous materials have developed rapidly in related fields.Nano-molecular sieve porous materials have made great progress in biosensor and detection,imaging and treatment because of their advantages of easy ion exchange,more active sites,good stability and non-toxicity.Especially in the synthesis and functional design of composite nanomaterials using zeolite as template,this paper presents a novel application prospect.In this paper,nanoscale EMT zeolites were synthesized by low-temperature non-template method.Then,Ag particles and CsPb Br₃ perovskite quantum dots were loaded in the framework of zeolites by ion exchange method.O n the basis of the above,we explore the application of tumor markers,humidity detection,cancer imaging,etc..Here,we also report the biosensor exploration of non-invasive disease detection of inorganic ordered porous materials.The results are as follows:[1]Electrochemical sensors based on Ag particle modified EMT molecular sieve composites:We first synthesized EMT molecular sieve by hydrothermal method without template at low temperature,then synthesized EMT molecular sieve loaded with Ag particles by silver ion exchange and triethylamine reduction.Glassy carbon electrode?glassy carbon electrode?based on Ag particle supported EMT molecular sieve composite was used as counter electrode,saturated KCl solution electrode as reference electrode,platinum electrode as working electrode.A highly sensitive and rapid detection of AFP,a marker of primary liver cancer,was carried out to detect the concentration of alpha-fetoprotein in the blood environment.The detection limit was as low as 30 pg/mL,and the linear range was 1 ng/mL-100ng/mL.The sensor had good stability.The initial response of 93.7%can be maintained after four weeks.[2]Perovskite Quantum Dots loaded EMT Molecular sieve Composites and their Cell Imaging:We first synthesized nanometer EMT zeolites,then replaced Na⁺in the pore cage of molecular sieve with Cs⁺.The molecular sieve containing cesium source was synthesized.The molecular sieve containing cesium source was then dissolved in octadecene,and reacted quickly with completely dissolved Pb X₂.Finally,EMT molecular sieve composite with perovskite modification,EMT-CsPbX₃,was obtained by washing.Compared with pure perovskite quantum dots,the composite has good stability.Due to the template effect of molecular sieve channels,a very small size perovskite quantum dot is obtained,and the luminescent properties of the perovskite quantum dot can be adjusted effectively because of the limited domain effect.Cytotoxicity test showed that the cells still had 80%activity at the concentration of150?g/mg.Fluorescence staining imaging of tumor cells was carried out by using the excitation wavelength of 405 nm of confocal microscope.[3]Gas sensors based on WO3/Au three-dimensional macroporous composite nanomaterials:A simple sacrificial template method was used to fabricate gas sensors based on3DIO WO₃/Au and pure WO₃ nanocomposites,and the gas sensing properties of acetone were systematically studied.The sensing performance of 3DIO WO ₃ was enhanced by modifying proper amount of Au nanoparticles.In addition,the"spillover effect"of au nanoparticles increased the reactive oxygen species and gas sensitivity of3DIO WO₃.The detection limit of acetone,a diabetic marker,was as low as 50 ppb,and the detection range was 50 ppb-100 ppm.It had good sensitivity,selectivity and stability,which can effectively and quickly distinguish between normal and diabetic patients.