Study On Preparation,quality Control And Pharmacology Effects Of Shenqi Yimu Cream

Objective:Taking Shenqi Yimu cream as research object,different extraction solvents and methods are used to optimize the preparation process according to the difference of physicochemical properties of active ingredients of Shenqi Yimu cream prescription.On the basis of optimizing the preparation process,the quality control of Shenqi Yimu cream is studied by modern instrumental analysis methods and techniques,and the effective active ingredients of Shenqi Yimu cream are studied from the aspects of TLC identification and content determination 4 kinds of medicinal herbs TLC identification and 2 kinds of the main active ingredient content determination method,the research results are expected to reach the advanced level of similar studies in China.And preliminary study of its hemostatic pharmacological effects,better for the Shenqi Yimu cream gynecological diseases used to provide experimental evidence.Methods:?1?Preparation process:Take different extraction solvents and methods to optimize the preparation process,so as to formulate the best preparation method of Shenqi Yimu cream.?2?Quality control:Thin layer chromatography of motherwort,radix astragali,etc.is established to establish the thin layer chromatography identification method of radix astragali and radix astragali by the quality standard of Shenqi Yimu Paste.The determination of leonurine hydrochloride.The chromatographic conditions are as follows:Xtimate^TM-C₁₈ column?4.6×250 mm,5?m?with a mobile phase of acetonitrile-0.01%phosphoric acid solution?15:85?at a flow rate of 1.0ml/min with a column temperature of 25°C and a detection wavelength of 280nm,injection volume of 10?L.Using the preparation of flavonoids pre-experimental reaction is positive characteristics,using UV spectrophotometry determination of total flavonoid content of Shenqi Yimu cream,to establish its content determination.?3?Pharmacological effects:determination of the mouse bleeding time and clotting time,determine the role of hemostasis pharmacological studies.Results:?1?Preparation process:The best preparation conditions of Shenqi Yimu cream are as follows:the optimum conditions of alcohol extraction are as follows:70%ethanol as solvent,8 times of ethanol,extraction for 3 times,1.5h each.Water to mention some of the best conditions is to add12 times the amount of water soak 60min,boiling 2 times,each 1.5h.?2?Quality control:Thin-layer chromatography is a simple and rapid method using the Chinese Pharmacopoeia 2015 edition.The spots obtained are clear and distinct.Test results reliable,accurate,with high value.In the experiment of the determination of leonurine hydrochloride by HPLC,the recovery rate of the sample is 99.87%and the RSD is 0.50%?n=6?,which shows that the method is simple,rapid and accurate.It can be used in hydrochloric acid leonurine content determination.The total flavonoid content of Shenqi Yimin cream is determined by UV spectrophotometry.The average recovery of the sample is 99.45%,RSD=0.34%?n=6?,high sensitivity,specificity and good reproducibility.?3?Pharmacological study:Shenqi Yi Mu cream can activate the blood activity index test,which can prolong the bleeding time and clotting time in mice.Conclusions:The optimum preparation process of Shenqi Yi Mu cream was determined.The TLC identification of Shenqi Yi Mu cream was systematically studied for the first time,and the method of UV visible spectrophotometry and HPLC determination were established,and the pharmacological action of Shenqi Yi Mu cream was preliminarily determined,which provided some experimental basis for clinical application.