Study On Mucosal Microbiome In Gastric Carcinogenesis

Objective:We describe mucosal microbial changes associated in gastric carcinogenesis,try to identify some bacterial species which represent the stages from gastritis to gastric cancer,and to explore by microbial components structure to predict the severity of atrophic gastritis.Patient and Methods:We performed 16S rDNA gene analysis of gastric mucosal samples from 32 cases including superficial gastritis(SG),atrophic gastritis(AG)&intestinal metaplasia(IM)and gastric cancer(GC)in the first affiliated hospital of Nanjing Medical University hospital from March 1,2017-March 1,2018,to determine mucosal microbiome dysbiosis across stages of GC.Results:We observed significant mucosa microbial dysbiosis in IM and SG subjects.Most abundent species are acinetobacter,actinobacillus,and bifidobacterium longum.Four IM-enriched bacterial taxa whose species identifications correspond to Prevotella tannerae,Bacillus acidicola,Candidatus Koribacter versatilis,Microbacterium maritypicum.Four GC-enriched bacterial taxa whose species identifications correspond to Helicobacter pylori,Prevotella melaninogenica,Haemophilus parainfluenzae,Peptostreptococcus.We performed a-diversity analysis to find that the diversity of SG group is lower than AG group.We performed metagenomeSeq analysis of three groups,and find that the amount of species between three goups is different,with SG group lower than the other two groups.Conclusion:Differences in microbial diversity and richness are obtained in gastric Carcinogenesis.We recommend Fusobacterium nucleatum,Streptococcus anginosus,Pasteurella,Methylobacterium,Xanthobacter and Bacillus rutilis as gastric cancer detection mark,and Prevotella tannerae as a mark to predict the severity of atrophic gastritis with intestinal.We also call for improve our oral environment to slow progression of atrophic gastritis with intestinal maybe,and therefore slow gastric cancer formation.