Study On The Function And Mechanism Of Long Non-coding RNA LINC01585 In Breast Cancer

Long non-coding RNAs(lncRNAs)have been recently identified as critical biomarkers for cancer tumorigenesis and progression.However,lncRNAs have been implicated in breast cancer(BC)progression through largely unknown.Thus,the present study aimed to examine the molecular mechanisms and the underlying functions of novel lncRNAs.We screened 12 candidate lncRNAs located in breast cancer susceptibility loci that have been previously identified by genome-wide association studies and were predicted to contribute to the risk of breast cancer.These lncRNAs were analyzed using a total of 46 pairs of breast cancer tissues and surrounding adjacent non-tumor tissues by quantitative real-time PCR(qRT-PCR)assay.Here,we found that LINC01585 was significant downregulated in breast cancer tissues.We next conducted RNA pull-down experiments to identify the proteins that bind with LINC01585 and to understand the biological activity of LINC01585.LINC01585 was found to be located in the nucleus and to bind with the NONO protein.Mechanistically,downregulation of LINC01585 resulted in the dissociation of NONO from LINC01585,followed by its interaction with CRTC.The resulting NONO/CRTC complex increased the transcription of CAMP/CREB target genes,which in turn promoted breast cancer growth.A series of discoveries suggested that LINC01585 has a potential value in anti-carcinoma therapy,and thus deserves further investigation.