The Preparation And Anti-Microbial And Anti-fouling Properties Assessment Of Composite Materials Based On Polydopamine Nanospheres

Biomedical materials are widely used in the biomedical area.However,the surfaces of the materials are known to cause biofouling due to the adhesion of proteins and bacteria,which triggers an infection.Some bacterium will multiply and form a protective biofilm in an accommodating environment,which leads to serious problems for human healthcare.Therefore,it is necessary to use a variety of methods to modify biomedical materials so that they can resist the adhesion of bacterium and protein,kill bacterium,and improve the biocompatibility of materials.The promising approachs can be exploited for applications in the field of biomedicine.In this paper,poly-dopamine nanospheres(PDANS)are formed by self-polymerization of dopamine(DA)in a water-alcohol mixed solvent at room temperature.Next,the Nano Silvers(AgNPs),polyethylene glycol(PEG)and N,N-dimethyl-N-octadecylbetaine(BS-18)are attached to the surface of the PDANS by covalent binding.The antibacterial and anti-protein dual functional biological materials are produced after respectively blending with poly(methyl methacrylate)(PMMA)and silicone rubber(SR),which are commonly used to polymer film-forming and medical catheter material,and their antibacterial activity and anti-protein property are evaluated lastly.The specific works are as follows:1?Monodisperse PDANS with an average diameter of 110 nm have been synthesized through a facile and low cost method using deionized water and alcohol mixed solvent at room temperature.The PDANS possess surface functional groups(-OH,-NH2),exhibiting an extraordinary versatile active nature.It is demonstrated that the PDANS could serve as an active template for the convenient synthesis of various nanostructures,e.g.,PDANS-AgNPs and PDANS-4kPEG-BS-18 nanostructures.No surface modification or special treatment is required for the synthesis of these nanostructures,which makes the fabrication process simple and very convenient.The micromorphology of the PDANS,PDANS-AgNPs are observed by scanning electron microscopy(SEM);The PDANS-4kPEG-BS-18 is determined by Fourier transforms infrared spectroscopy(FT-IR),Zeta potential.Moreover,preliminary antibacterial assays indicate that these PDANS-AgNPs show enhanced antibacterial activities against Escherichia coli(Gram-negative bacteria)and Staphylococcus aureus(Gram-positive bacteria),primary antifouling assays manifest that these PDANS-4kPEG-BS-18 can resist the adhesion of protein,while they do not show significant in vitro cytotoxicity against HL-7702 human normal liver cells.2?The antibacterial and anti-protein dual functional materials are produced after respectively blending with PMMA and SR,which are maintained good chemical and mechanical stability.The modified materials are tested for using common methods such as antibacterial,antimicrobial adhesion,anti-protein adhesion,MTT test to evaluate their antibacterial activity and anti-protein property.The results show that the modified biomaterials not only have strong bactericidal,anti-protein and bacterial adhesion effects,but showed excellent cytocompatibility towards towards normal human liver cells,which greatly increase their application potential in the field of biomedicine.showed excellent cytocompatibility towards nonnal human liver...