Application And Research Of UW Solution In Replantation Of Rat Limb

ObjectiveThe purpose of this study is to explore the application of UW solution in the replantation of the rat limb,and to explore a simple method for the ideal preservation of the amputated limbs.Methods120 healthy adult male SD rats,weight 320-350g,were randomly divided into 8 groups:A,B,C,D,E,F,G,H.The isolated limbs of the Control Group A,C,E,G were cryopreserved in refrigerator at 4℃,while those of the Experiment Group B,D,F,H were preserved under the same condition after being perfused with UW solution.All the severed limbs were replanted back to the rats after different time length of cryopreservation:A,B 12 hours,C,D 20 hours,E,F 28 hours and G,H 36 hours.During the operations the femoral artery and venous patency were detected immediately after anastomosis.And after the operations the survival rates of limb replantation were observed.On the third day after the limb replantation,the tibial posterior muscle tissue was examined for pathology.The cell areas in pathological photographs were compared with Image analysis software Image-pro Plus 6.0 and the ultrastructural changes of skeletal muscle tissue were observed with electron microscope.ResultsThe femoral artery and venous patency in Experiment Group A,C,E and Control Group B,D,F are all 100 percent.However,in the Group G,H the severed limb vessels changed obviously,the patency dropped markedly and the survival rates of limb replantation turned out to be 0.The Experiment Groups perfused with UW solutions,had relatively higher survival rates compared with the Control Groups(12h 93.33%VS 53.33%;20h 80%VS 40%,28h 53.33%VS 13.33%,P<0.05)and the skeletal muscles edema was lighter than that of the Control Group,with a larger proportion of cell areas(12h 69.49 ±8.57 VS 44.75 ±6.34 20h 56.34 ±7.73 VS 36.69 ± 5.74 28h 45.76± 8.23 VS 31.49 ±7.72 P<0.05).There was a significant difference.Electron microscope observation showed that the Experiment Group bore a lighter damage of myofibril,interstitial tissue and mitochondria compared with the Control Group.ConclusionThe combination of UW solution and cryopreservation of organs is an ideal and proper way to preserve the amputation limb,to extend the time limit for replantation,and to achieve a better survival results of limb replantation.Besides,UW solution is easy to be promoted in the treatment of isolated limb in clinical work.