Expression Of MiR-200c In Pancreatic Cancer And Its Clinical Significance

Background:Pancreatic carcinoma(PC)is a common malignancy worldwide.Because of its special physiological location and the biological characteristics of pancreatic cancer,early diagnosis of pancreatic carcinoma is very difficult.The metaphase progress is rapid,the late prognosis is poor,and the mortality rate is very high.The five-year survival rate is generally not more than 5%.The clinical treatment is based on surgery,but the early symptoms of pancreatic cancer are non-specific.Once the symptoms are diagnosed,they often enter the middle and late stage.The time of surgical treatment is lost,the radiotherapy is poor,and chemotherapy is easy to produce multiple drug resistance.It is known as “Cancer King”.It is called.It is generally believed that chronic pancreatitis is unrecoverable,long-term high-fat diet causes excessive body weight is a major risk factor for pancreatic cancer,diabetes,long-term alcohol and smoking also have a certain close relationship with the occurrence of pancreatic cancer.The onset of pancreatic cancer may be related to genetics and living conditions.Big data show a certain family genetic predisposition.Ranked fourth in the United States for cancer-related causes of death,the 5-year survival rate is less than 5%,and the morbidity and mortality rates have been rising year after year,posing a serious threat to human health.Therefore,in order to improve the prognosis of patients,we need to further study the pathogenesis and process of pancreatic cancer,explore new diagnostic indicators,and provide new methods for early diagnosis and treatment of pancreatic cancer.In recent years,as a large number of researchers have conducted in-depth studies in molecular biology and other fields,a number of emerging molecular basic disciplines have developed rapidly.A large number of unknown microRNAs(miRNAs)have been discovered and recognized by humans and have good specificity in the future.It may be one of the effective methods for clinical diagnosis and treatment of pancreatic cancer.MicroRNAs(miRNAs)are single-stranded nucleotides of about 22 nt in length and do not participate in the coding synthesis of encoded proteins.They are a type of noncoding RNA(ncRNA).In the past,people thought that miRNAs were only small,non-meaningful fragments in human gene fragments,and they could not produce a role in human gene expression and inheritance.They were also called “dark matter”.With the deepening of the Human Genome Project,the researchers were surprised to find that the number of these miRNA-associated DNAs was much higher than that of the coding RNA,the messenger RNA(mRNA).For this phenomenon that is contrary to the use of biological waste,researchers from around the world have conducted more extensive research on miRNAs and found that the higher the biological evolution,the more miRNA content and species are more abundant.This also shows that miRNA does not seem to be useless,but is temporarily undiscovered and understood.With the advancement of experimental techniques and research methods in recent years,researchers have conducted more in-depth studies on miRNAs and found that miRNAs can regulate the expression of more than 60% of human protein-coding genes,and they are also widely involved in various aspects of life phenomena,such as growth.Development,apoptosis,immunity,etc.,play an important regulatory role in the pathogenesis of the disease and even the formation of tumors.Current research shows that the detection of miRNA levels can be used to distinguish between tumor and normal tissues,which can help early stage diagnosis.Up-regulation or down-regulation of miRNA expression can accelerate or delay the progression of tumors.It has certain significance for the treatment of tumors;Differential expression can also be used to judge patients' clinical outcomes.Therefore,in-depth study of miRNA expression and function in tumors is of great significance for studying tumor biology.In recent years,the miR-200 family has received extensive attention in tumor research.Many studies show that miR-200 c,one of miR-200 family members,has a wide range of tumor regulation effects on lung cancer,colorectal cancer,kidney cancer,ovarian cancer,and bladder cancer.All have an effect,but the expression of miR-200 c in pancreatic cancer tissues,the role of cancer promotion or tumor suppressor and their functions are not yet clear.Objective:To detect the expression of miR-200 c in pancreatic cancer tissues and paracancerous tissues,analyze the relationship between miR-200 c expression and clinical pathological features in pancreatic cancer,and to investigate the role of miR-200 c in the development of pancreatic cancer.significance.Methods:A total of 56 pancreatic cancer tissues and their matched paracancerous tissues were surgically resected from January to December 2016 in Zhengzhou University Affiliated Tumor Hospital.RT-PCR was used to detect miR-200 c in pancreatic cancer.Tissue and paracancerous tissue expression.According to the relative expression level of miR-200 c in pancreatic cancer tissues,the median expression was used to divide into high expression group and low expression group,and the clinicopathological characteristics(sex,age,tumor size,tumor location,tumor differentiation,TNM stage)were analyzed.,CA199 levels,lymph node metastasis were compared to analyze the relationship between the differential expression of miR-200 c in pancreatic cancer tissues and the characteristics of clinical cases,and to explore the significance of miR-200 c in the development of pancreatic cancer.Results:1.The expression of miR-200 c in pancreatic cancer tissues: The relative expression of miR-200 c in pancreatic cancer tissues(0.37±0.06)was significantly lower than that in adjacent tissues(P<0.05).2.Analysis of miR-200 c and clinicopathological characteristics of pancreatic cancer: The relative low expression of miR-200 c in pancreatic cancer was significantly correlated with lymph node metastasis(P=0.001)and TNM stage(P=0.029),but related to gender,age,and tumor size.There was no significant correlation between tumor location,tumor differentiation,and CA199 levels(P>0.05).Conclusion:1.The expression of miR-200 c in pancreatic cancer tissues was significantly lower than that in adjacent tissues,suggesting that miR-200 c may play a role in tumor suppressor genes in pancreatic cancer.2.The low expression of miR-200 c is closely related to lymph node metastasis and tumor TNM stage in pancreatic cancer,suggesting that miR-200 c may be involved in the development,progression and metastasis of pancreatic cancer.