Correlation Analysis Of Rs1695,Smoking And Lung Cancer

Objective:The paper is to explore relations between polymorphism of GSTP1 genetic locus rs1695 and susceptibility to lung cancer in Chinese Han population.Besides,stratification analysis is conducted on the basis of age,gender,smoking status,tumor-related family history and pathological type to observe relations between polymorphism of GSTP1 genetic locus rs1695 and susceptibility to lung cancer in the subgroups.Methods:The method of case-control study was adopted.A total of 974 lung cancer patients who were diagnosed with pathology and 1005 physical examination-based healthy cases were enrolled from Han population in Shanghai and Taizhou of Jiangsu Province.Samples of peripheral venous blood were collected from the subjects,Blood Genomic DNA Mini Preparation Kits were used to extract DNA,and SNPscanTM was used to obtain genetic types of GSTP1 genetic locus rs1695.Genotyping is based on a Quality Control(QC)flow,involving a successful detection rate of above 95%,repeated detection of genotype,test of internal positive control samples,and Hardy-Weinberg genetic equilibrium test.Student t-test and Chi-square test were used to compare differences of age,gender,smoking status and tumor-related family history between the case group and the control group as well as A and G allele frequencies of GSPT1 genetic locus rs1695 and distributions of AA,GA and GG genotypes frequencies between the two groups.Multivariate logistic regression analysis was applied to analyze correlations between allele model,genotype model,dominant gene model and recessive gene model and the risk of lung cancer.Besides,data were adjusted for age,gender,smoking status and tumor family history of the subjects.The results were expressed as odds ratios(ORs)and 95%confidence intervals(CIs).SPSS 19.0 statistical software was used for statistical analysis,all p values were based on two-sided test,and p<0.05 indicates statistical significance.Results:1.A total of 974 lung cancer patients and 1005 healthy cases were enrolled in the study,and showed no statistically significant differences of gender(p=0.101)and age(p=0.944).The amounts of smoker and individual with positive family history of tumor were both statistically higher in the lung cancer group than the control group(p<0.001).2.The detection rate of genotype in the study was 99.9%.The frequencies of genotypes at A/G locus of GSTP1 rs1695 were consistent with Hardy-Weinberg genetic equilibrium(p>0.05).3.The lung cancer group and the control group presented no statistically significant differences of A and G allele frequencies(?2 = 3.785,p = 0.053),while individuals with G allele had reduced the risk of lung cancer(OR = 0.811,95%CI 0.684-0.961,p = 0.016).There was no significant difference of genotype distribution frequency between the two groups(?2 = 4.072,p = 0.131).GG genotype reduced the risk of lung cancer(OR = 0.591,95%Cl 0.347-0.988,p = 0.048),while GA genotype showed no obvious correlation with the risk of lung cancer(OR = 0.838,95%CI 0.683-1.028,p = 0.091).4.It is indicated by stratification analysis that the risk of lung cancer in G allele carriers was reduced in male(OR = 0.784,95%CI 0.639-0.962,p = 0.020),individuals with smoking history(OR = 0.732,95%CI 0.591-0.906,p = 0.004),individuals without positive family history of tumor(OR = 0.782,95%CI 0.643-0.950,p = 0.014)and patients with adenocarcinoma(OR = 0.798,95%CI 0.641-0.990,p = 0.042).In females,individuals without smoking history,individuals with positive family history of tumor,patients with squamous carcinoma and patients with small cell lung cancer,there was no obvious relation between G allele and the risk of lung cancer(p>0.05).Conclusion:The polymorphism of GSTP genetic locus rs1695 is related with the risk of lung cancer,and is expected to be a target spot for prediction of lung cancer.